PBS buffer stands for phosphate buffered saline, which combines the following components: sodium chloride for isotonicity, potassium chloride, and Na₂HPO₄ and NaH₂PO₄ as phosphate buffers. It has a pH of approximately 7.4, which is acceptable for many biological tasks. In addition, other body fluids, as well as normal human blood, have a comparable pH making it an outstanding reagent in all biological applications such as cell culture or protein assays.
Let us see in this blog why PBS is one of the indispensable reagents in industrial biotechnologies.
Key Applications of PBS in Biology
The Phosphate Buffer Solution is much appreciated since it has many applications in biology and biotechnology. The applications include cell culturing practices such as washing cells, removing media and holding internal normal conditions during experiments. Balance of pH and osmotic level ensures life of cells for a longer time without compromising any results of experiments.
- Immunoassays
A wash buffer in most immunoassays, such as those requiring blotting, is PBS, allowing for standardizing and lowering the non-specific binding of the immunoassays, hence making them more reliable in diagnosis and pharmaceutical testing.
- Molecular Genetics
PBS is used in DNA and RNA extraction and preservation. It works not only as an additive, but also prevents destabilization due to degradation during handling and storage, keeping integrity throughout any further analysis.
Why PBS is Important to Biotech Companies?
These are the following lists of advantages that PBS offers concerning biotechnology companies.
- Reliability: PBS in biology gives osmotic protection and maintains the pH of biological samples that ensure all experiments where PBS is used returns valid outcomes.
- Versatility: This is a variety of applications of PBS in biotechnology like cell culture, protein assays, and DNA that indeed were irreplaceable in any biotech laboratory.
- Economical reagent: PBS preparation is very simple, isn’t too expensive, and thus all the time it is economical for companies worldwide in biotechnology.
Conclusion
Phosphate buffer saline is the backbone of biotechnology in various ways. It guarantees the credibility and accuracy of all results through the method of sustaining cells and stabilizes biological samples under experimental conditions from start to finish. From cell culture, protein analysis, and genetic research, in fact, PBS is ever present at the very heart of laboratory methodologies for the entire biotech industry. Connect with PurMa Biologics LLC by finding out more.
