The chemicals used determine the outcomes obtained in cell culture experiments to a large extent. One of the most frequently used reagents during biotechnological procedures is Dulbecco Phosphate-Buffered Saline. This chemical plays a big part in different media like Dulbecco Medium and DMEM and is applied in a wide range of lab experiments. In this blog, let’s shine a light on the best practices for the application of Dulbecco PBS in cell culture experiments.
Role of Dulbecco PBS in Cell Culture Experiments
Dulbecco PBS is a type of PBS that is created to mimic the ionic makeup of the human body while providing cells with the ideal environment in nongrowth experiments. This reagent is also used to wash cells to remove any enzymes or extra serum without harming the integrity of the cells. Before the procedures like cell transfer and detachment, this step is essential.
If correctly used, Dulbecco PBS maintains the osmotic equilibrium, which prevents cells from shrinking and expanding. This step ensures cell viability and retains its biological characteristics during crucial procedures. When Dulbecco medium is combined with DMEM, it acts as a whole system for preserving cell health and performance.
Best Practices for Using Dulbecco PBS
- Right Temperature
Dulbecco PBS should always be at the right temperature for cells that are going to be used in the experiment.
- Balanced pH
The pH of Dulbecco PBS should be between 7.2 and 7.4; deviations from this range might cause unwanted stress to the cells and can affect the total outcome of the experiment.
- Proper Handling:
Always take care to pipette them gently while washing them in Dulbecco PBS, as any kind of bodily harm to the cells is very dangerous.
⦁ Appropriate Volume Usage
It is highly important to use just the right amount of Dulbecco PBS to cover the monolayer of the cells without adding much more. Too little or too much will be inadequate in removing unwanted residues or diluting the reagents.
Pairing With DMEM
Dulbecco DMEM, is one of the most important media used in cell culture techniques as this media is nutrient-rich and promotes cell development. Cleaning cells with Dulbecco PBS before placing them on DMEM for culture can be highly beneficial, as it will make sure to restrict any unwanted components to manipulate the results of the experiment.
Conclusion
Dulbecco PBS stays a fundamental reagent for most cell culture techniques as it provides cells with a safe and stable environment when handling. With the best practices in procedures, you are more likely to get the best results for your research in terms of healthy and viable cell cultures. The combination of Dulbecco PBS with Dulbecco modified eagle medium provides more healthy and viable cell cultures.
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