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Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer

Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer contains L-Alanyl-L-Glutamine as well as serum-reducing ingredients. Additionally, Advanced BME needs 2-5% FBS and it furthermore provides more consistency.

Description

Background

PurMaTM Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in Advanced BME. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.

Contents

Advanced BME in HBSS Contains:

  • It includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-BME incorporates elements to significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems, which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced BME (CD-A-BME) is the outcome of decades of cutting-edge research.  Furthermore, it will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

The complete formulation can be found here: Advanced BME in Hanks’ Buffer (HBSS) Formulation

References

  1. Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, Teleki S, von Seefried A, Walton MJ, Macmorine HG. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
  2. Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909

 

ParameterSpecification
AppearanceRed, clear liquid
pH7.2 ± 0.1
Osmolality275-360 mOsm/L
EndotoxinNMT< 2EU/mL
MycoplasmaNegative
Suitability Suitable for mammalian cell culture
AdditiveSodium pyruvate
IndicatorPhenol red
Mycoplasma DetectionNegative
Sterility TestedSterile filtered using 0.22 µm filter
FormLiquid
Shipping Condition Room temperature

 

Additional information

Condition

A-BME (HBSS) Standard Formulation, A-BME (HBSS) w/o L-Alanyl-L-Glutamine, A-BME (HBSS) w/o Phenol Red, A-BME (HBSS) w/o Sodium Bicarbonate, A-BME (HBSS) w/o Glucose, A-BME (HBSS) w/o L-Alanyl-L-Glutamine , w/o Phenol Red, A-BME (HBSS) w/o L-Alanyl-L-Glutamine , w/o Phenol Red, w/o Sodium Bicarbonate, A-BME (HBSS) 15 mM HEPES (3.6 g/L), A-BME (HBSS) 25 mM HEPES (5.9 g/L), A-BME (HBSS) With Sodium Pyruvate (0.11 g/L), A-BME (HBSS) High Sodium Bicarbonate (0.85 g/L), A-BME (HBSS) High Glucose (4.5 g/L), A-BME (HBSS) 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L), A-BME (HBSS) 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (0.85 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Quality Control

[vc-quality-control-tab]

Formulation

Advanced BME in Hanks’ Buffer (HBSS)

 

SDS

SDS

1 review for Advanced Basal Medium Eagle (A-BME) in Hanks’ Buffer

  1. azadi@live.ca

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

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