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Advanced Dulbecco's Modified Eagle Medium/F-12 (A-DMEM F-12)

Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12)

Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12) needs 2-5% FBS and provides more consistency.

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Description

Background:

PurMa™ Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes.  In Advanced DMEM F-12, L-Alanyl-L-Glutamine is used as the source of glutamine.  Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

A-DMEM F-12 Contains:

  • Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12) contains all the twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • It includes the elements which significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Additionally, A-DMEM F-12 is a significant step toward a chemically defined form of this media. Undoubtedly, Chemically Defined DMEM F-12 (CD-DMEMF-12) is the outcome of decades of cutting-edge research. CD-DMEM F-12 will soon be offered globally to laboratories who are looking for consistency and repeatability among their data.

Formulation: Complete formulation is available here: Advanced DMEM-F12 Formulation

References:

  1.  Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016 /0042-68 22(59)90043-1. PMID: 13669362.
  2. Plaque production by the polyoma virus. DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 1366936.
  3. Evaluation of a simple protein free medium that supports high levels of monoclonal antibody production. Qi et al. 1996 Jun;21(2):95-109. doi: 10.1007/BF02215660. PMID: 22358660.
ParameterSpecification
AppearanceRed, clear liquid
pH7.2 ± 0.1
Osmolality275-360 mOsm/L
EndotoxinNMT< 2EU/mL
MycoplasmaNegative
Suitability Suitable for mammalian cell culture
AdditiveSodium pyruvate
IndicatorPhenol red
Mycoplasma DetectionNegative
Sterility TestedSterile filtered using 0.22 µm filter
FormLiquid
Shipping Condition Room temperature

 

Additional information

Condition

A-DMEM F-12 Standard Formulation, A-DMEM F-12 Standard Formulation, ATCC Formulation, A-DMEM F-12 w/o L-Alanyl-L-Glutamine, A-DMEM F-12 w/o Phenol Red, A-DMEM F-12 w/o Sodium Bicarbonate, A-DMEM F-12 w/o Sodium Pyruvate, A-DMEM F-12 w/o Glucose, A-DMEM F-12 w/o Lipoic Acid, A-DMEM F-12 w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, A-DMEM F-12 w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-DMEM F-12 w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-DMEM F-12 Low Glucose (1.5 g/L), A-DMEM F-12 Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 15 mM HEPES (3.6 g/L), A-DMEM F-12 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 w/o Sodium Pyruvate, w/o phenol Red, A-DMEM F-12 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), A-DMEM F-12 w/o Phenol Red, Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.2 g/L), A-DMEM F-12 High Glucose (4.5 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

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