Advanced Glasgow’s Modified Eagle’s Medium – PurMa Biologics LLC
Advanced Glasgow’s Modified Eagle’s Medium is free of glutamine contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced Glasgow’s medium needs 2-5% FBS and provides more consistency.
Description
Background
PurMaTM Advanced Glasgow’s Modified Eagle’s Medium is free of glutamine amino acids. Moreover, it contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-GMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced Glasgow’s Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-GMEM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Glasgow’s Modified Eagle’s Medium is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Glasgow’s medium (CD-Glasgow’s meium) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced Glasgow’s Formulation
References
- The stimulation of Na+, K+-ATPase activity in the medulla of the rat kidney by [arginine] vasopressin and its analogues. Pippard C, Baylis PH.Clin Sci (Lond). 1984 May;66(5):561-7. doi: 10.1042/cs0660561.PMID: 10.1023/a:1017989305873. PMID: 15348233.
- Induction of dopamine-releasing cells from primate embryonic stem cells enclosed in agarose microcapsules. Ando T, Yamazoe H, Moriyasu K, Ueda Y, Iwata H.Tissue Eng. 2007 Oct;13(10):2539-47. doi: 10.1089/ten.2007.0045.PMID:
- Uptake of glutamate, not glutamine synthetase, regulates adaptation of mammalian cells to glutamine-free medium. McDermott RH, Butler M.J Cell Sci. 1993 Jan;104 ( Pt 1):51-8. doi: 10.1242/jcs.104.1.51.PMID:
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A- GMEM Standard Formulation, A- GMEM w/o Phenol Red, A- GMEM w/o Sodium Bicarbonate, A- GMEM w/o Sodium Pyruvate, A- GMEM w/o Glucose, A- GMEM Low Glucose (1.5 g/L), A- GMEM Low Sodium Bicarbonate (1.1 g/L), A- GMEM 15 mM HEPES (3.6 g/L), A- GMEM 25 mM HEPES (5.9 g/L), A- GMEM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), A- GMEM 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.1 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
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