Advanced MEM-Alpha with Nucleosides in Earle’s Buffer
Advanced-MEM-Alpha with Nucleosides in Earle’s Buffer (A-MEM-a-N-EBSS) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. More importantly, Advanced MEM-α-N needs only 2-5% FBS and provides more consistency.
Description
Background
PurMaTM Advanced-MEM-Alpha with Nucleosides (A-MEM-α-N) in Earle’s Buffer contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. In A-MEM-α-N, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced MEM-α-N in EBSS Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-MEM-α-N incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
PurMa™ Advanced MEM-α with nucleoside (A-MEM-α-N) is modified from Advanced MEM-α with the following ingredients:
- Ribonucleosides
- Deoxyribonucleosides
Consequently, Advanced-MEM-Alpha with Nucleosides (A-MEM-α-N) in Earle’s Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM-α-N (CD-A-MEM-α-N) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced MEM alpha with Nucleoside in Earls Buffer
References
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
- Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, et al. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
- Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham’s F-10. Roudebush WE, et al. J Assist Reprod Genet. 1994 Apr;11(4):203-7. doi: 10.1007/BF02211809. PMID: 7711383.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-MEM-α-N Standard Formulation, A-MEM-α-N w/o L-Alanyl-L-Glutamine, A-MEM-α-N w/o Phenol Red, A-MEM-α-N w/o Sodium Bicarbonate, A-MEM-α-N w/o Glucose, A-MEM-α-N w/o Lipoic Acid, A-MEM-α-N w/o L-Alanyl-L-Glutamine , w/o Phenol Red, A-MEM-α-N w/o L-Alanyl-L-Glutamine , w/o Sodium Bicarbonate, A-MEM-α-N w/o L-Alanyl-L-Glutamine , w/o Phenol Red, w/o Sodium Bicarbonate, A-MEM-α-N 15 mM HEPES (3.6 g/L), A-MEM-α-N 25 mM HEPES (5.9 g/L), A-MEM-α-N w/o Sodium Pyruvate, A-MEM-α-N High Glucose (4.5 g/L), A-MEM-α-N Low Sodium Bicarbonate (0.35 g/L), A-MEM-α-N Low Sodium Bicarbonate (0.85 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
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