Sale!
Advanced 1640 (A-RPMI-1640)

Advanced Roswell-Park Memorial Institute 1640 (A-RPMI-1640)

(11 customer reviews)

Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-RPMI-1640 needs 2-5% FBS and provides more consistency.

SKU: N/A Categories: Roswell-Park Memorial Institute 1640, Cell Culture Media Tags: Cell Culture, Advanced, RPMI 1640, L-Alanyl-L-Glutamine, Serum Reduction, Human Transferrin, Recombinant Insulin

Description

Background

PurMaTM Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-RPMI-1640, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Contents

Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-RPMI-1640 incorporates the elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents Improperly iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Additionally, Advanced Roswell Park Memorial Institute 1640 (A-RPMI-1640) is a significant step toward a chemically defined form of this media. Chemically Defined Advanced RPMI-1640 (CD-A-RPMI-1640) is the outcome of decades of cutting-edge research. It will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here:  Advanced RPMI-1640 Formulation

References

  1. Culture of normal human leukocytes. Moore GE, et al. 1967 Feb 20;199(8):519-24. PMID: 4960081
  2. Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. Dunne HW, et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.
  3. Long-term culture of human leukaemic leukocytes. Pössnerová V, et al. 1970;17(5):513-23. PMID: 5274436
Parameter Specification
Appearance Red, clear liquid
pH  7.2 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  Sodium pyruvate
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A-RPMI-1640 Standard Formulation, A-RPMI-1640, Standard Formulation (ATCC formulation), A-RPMI-1640 w/o L-Alanyl-L-Glutamine, A-RPMI-1640 w/o Phenol Red, A-RPMI-1640 w/o Sodium Bicarbonate, A-RPMI-1640 w/o Glucose, A-RPMI-1640 w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-RPMI-1640 w/o Phenol Red, w/o Sodium Bicarbonate, A-RPMI-1640 w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-RPMI-1640 With Low Glucose (1 g/L), A-RPMI-1640 With High Sodium Bicarbonate (3.7 g/L), A-RPMI-1640 With 15 mM HEPES (3.6 g/L), A-RPMI-1640 With 25 mM HEPES (5.9 g/L), A-RPMI-1640 With 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), A-RPMI-1640 With 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L, A-RPMI-1640 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), A-RPMI-1640 w/o Phenol Red, with 25 mM HEPES (5.9 g/L), A-RPMI-1640 w/o Phenol Red, High Sodium Bicarbonate (3.7 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

11 reviews for Advanced Roswell-Park Memorial Institute 1640 (A-RPMI-1640)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

Related products

  • Advanced Dulbecco’s Modified Eagle Medium/F-12 (A-DMEM F-12) Quick View
  • McCoy’s 5A Modified Media (M5AMM) Quick View
  • Cell Culture Suited Vitamin Solution (100X) Quick View