Advanced Williams’ E Medium
Advanced Williams’ E Medium contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-Williams’ E needs 2-5% FBS and provides more consistency.
Description
Background
PurMaTM Advanced Williams’ E Medium contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. This results in less variation amongst lot-to-lot serum changes. In A-Williams’ E, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced Williams’ E Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-Williams’ E incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Williams’ E Medium is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced Williams’ E (CD-A-Williams’ E) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available: Advanced Williams’ E Medium Formulation
References
- Isolation and long-term cell culture of epithelial-like cells from rat liver. Williams GM, et al. Exp Cell Res. 1971 Nov;69(1):106-12. doi: 10.1016/0014-4827(71)90316-8. PMID:
- Primary in vitro antibody responses by purified murine B lymphocytes in serum-free defined medium. Mosier DE. J Immunol. 1981 Oct;127(4):1490-3. PMID: 6792277
- Serum-free culture of rabbit meniscal fibrochondrocytes: proliferative response. Webber RJ, Zitaglio T, Hough AJ Jr. J Orthop Res. 1988;6(1):13-23. doi: 10.1002/jor.1100060103. PMID: 333473
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-William’s E Standard Formulation, A-William’s E w/o L-Alanyl-L-Glutamine, A-William’s E w/o Phenol Red, A-William’s E w/o Sodium Bicarbonate, A-William’s E w/o Sodium Pyruvate, A-William’s E w/o Glucose, A-William’s E w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-William’s E w/o Phenol Red, w/o Sodium Bicarbonate, A-William’s E w/o L-Alanyl-L-Glutamine , w/o Phenol Red, w/o Sodium Bicarbonate, A-William’s E High Glucose (4.5 g/L), A-William’s E Low Sodium Bicarbonate (1.1 g/L), A-William’s E 15 mM HEPES (3.6 g/L), A-William’s E 25 mM HEPES (5.9 g/L), A-William’s E 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), A-William’s E 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.1 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
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