Leibovitz’s L-15 Medium
Leibovitz’s L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.
Description
Background
Leibovitz’s L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH.
This media contains:
- High Level of Sodium pyruvate (5mM)
- Undoubtedly, the absence of Sodium Bicarbonate makes this media unique.
- Basically, the buffering Capacity is maintained by other elements, mainly phosphates.
- And finally Using Galactose instead of glucose makes this media quite different.
Application of Leibovitz’s L-15
- Cryopreservation of cells and tissues such as Xenopus sperm and embryonic cells.
- Establishing human tumor cells lines such as Hep-2 cells.
- As a supplement in sterile cell media for the dissection and isolation of kidney cells.
- As an important media which provides proper buffering system for culturing of lung cells.
- A valid option for growing fish originated from cell lines.
PurMa Biologics manufactures 107 types of Leibovitz’s L-15
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments, and the result of your lab. Considering the highest quality, our prices are extremely reasonable.
Formulation
For complete formulation please visit the “formulation tab”, as well as by clicking here: Leibovitz’s L-15 Formulation
References
- The effect of a phorbol ester on amphibian myogenesis. Zeng MB, Tseng MP. Shi Yan Sheng Wu Xue Bao. 1990 Mar;23(1):106-15. PMID: 2382522.
- Expression of differentiation molecules is preserved in human fetal kidneys during culture. Brière et al. Acta Histochem. 1990;89(2):157-66. doi: 10.1016/s0065-1281(11)80351-x.PMID: 2093265.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | Leibovitz's L-15 Standard Formulation, Leibovitz's L-15 w/o Glutamine, Leibovitz's L-15 w/o Phenol Red, Leibovitz's L-15 w/o Sodium Pyruvate, Leibovitz's L-15 w/o Galactose, Leibovitz's L-15 High Galactose (4.5 g/L), Leibovitz's L-15 15 mM HEPES (3.6 g/L), Leibovitz's L-15 25 mM HEPES (5.9 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
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