Mycoplasma Growth Media
PurMa™ Mycoplasma Growth Media is a proprietary combination. This media does not only provide an excellent environment for various species of mycoplasma to grow, but is also used for detecting the exact strain of contaminating mycoplasma.
Description
PurMa™ Mycoplasma Growth Media
PurMa™ Mycoplasma Growth Media is a proprietary combination which provides an excellent environment for various species of mycoplasma to grow.
Nutrition
Mycoplasma has a complex nutritional requirement. This therefore, makes them dependent on external supplies. PurMa™ Mycoplasma Growth Media is ideal for growing different strains. Mycoplasmas have three important features: they are categorized as Mollicutes class, they also possess low GC content, and they basically don’t have cell wall. Additionally, mycoplasma has a simplified metabolic pathway and therefore it is a hard to grow this organism, especially under laboratory conditions.
Mycoplasma Detection
PurMa™ Mycoplasma Growth Media is also used for detecting the exact strain of contaminating mycoplasma.
The best tool for evaluating the presence of mycoplasma in any liquid such as media is by using the PCR method. The mycoplasma agar bcan also be used by growing mycoplasma and counting mycoplasma fried egg” shape colonies.
- Initially, Incubate the contaminated samples of media, FBS or cell line in 5 ml of PurMa™ MycoGrowth media (liquid). The condition must be anaerobic while shaking.
- Pipet 100 µl of the media on an PurMa™ MycoAgar plate.
- After three days individual fried egg structure will start to show up.
- Pick up several colonies and grow them in PurMa™ MycoGrowth liquid media.
- Use PurMa™ Mycoplasma PCR Detection kit (Cat#: P5M011404) to amplify the fragment.
- Have the PCR reaction cleaned and sent for sequencing.
- The 230bp PCR fragment resulted from our kit will clearly determine the strain of your contaminant.
Special Features
- Easy -to- Use
- Is an excellent tool to determine your exact mycoplasma contamination.
- Contains a proprietary formulation which maximizes the growth of all known mycoplasmas.
- Rigorously tested for bacteria, endotoxin, and mold.
- Free of all mycoplasmas inhibiting growth elements.
References
- DEFINED MEDIUM FOR MYCOPLASMA LAIDLAWII. TOURTELLOTTE et al. J Bacteriol. 1964 Jul;88(1):11-5.
- Comparison of undefined medium and its dialyzable fraction for growth of Mycoplasma. Pollock et al. 1969 Feb;97(2):522-5.
| Parameter | Specification |
| Appearance | Liquid/Powder |
| pH | 7.4 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Indicator | NA |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid/ powder |
| Shipping Condition | Ambient |
Additional information
| Format | MycoAgar, MycoGrowth |
|---|---|
| Size | 500 g, 1 kg, 1 x 500 ml, 1 x 1000 ml |
Quality Control
[vc-quality-control-tab]11 reviews for Mycoplasma Growth Media
Only logged in customers who have purchased this product may leave a review.
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azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 21-Feb-2025 15:30:00
Ease of placing the order: ‘not given’
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 24-Feb-2025 13:50:14
Ease of placing the order: ‘not given’
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 27-Feb-2025 17:42:44
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 16:31:44
Ease of placing the order: ‘not given’
michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 17:29:13
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 10-Mar-2025 14:32:38
Ease of placing the order: ‘not given’
jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5