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Mycoplasma Treatment Reagent (MTR) (100X)

Mycoplasma Treatment Kit (MTK) (100X)

(11 customer reviews)

PurMa™ Mycoplasma Treatment Kit (MTK) (100X) is a valuable tool for eliminating mycoplasma from live cells. PurMa™ MTRI, PurMa™ MTRII as well as PurMa™ MTRIII

 

SKU: N/A Category: Mycoplasma Detection and Elimination Reagents Tags: Mycoplasma Treatment, Removing From Live Cells

Description

PurMa™ Mycoplasma Treatment Kit (MTK) (100X) is a valuable tool for eliminating mycoplasma from live cells.

Important Note

Completing the eradication of mycoplasma from a contaminated cell line is laborious, time consuming as well as expensive. The procedure might also cause more contamination. So, we strongly recommend disposing of the contaminated cell line and starting a new culture if possible. This, however, might not be possible due to high value cell lines such as monoclonal antibodies or stable cell lines.

Feeder Cells

During the eradication process, fresh spleen cells of a healthy mouse are needed. Before starting the process:

Isolate the spleen cells from a healthy adult mouse.

Resuspend in 10 ml media of choice containing 20% FBS in a 15ml snap tube and place in 37°C CO2 incubator.

For detailed procedure regarding the isolating spleen cells, click here: Detailed Procedure

before you start

  1. Decontaminate your CO2 incubator, Hood, and benches before starting the procedure, otherwise in no time, the contamination will be back into your cultured cells.
  2. Use unopened media bottles and fresh FBS.
  3. During the following mentioned procedure and after that, wear mask at all times while you work in cell culture lab.
  4. Spray your gloves, bench, hood with PurMa™ Mycospray (1X) (Cat#: P5M010405) before and after work. PurMaTM Mycospray (1X) instantly eliminates/ deactivates mycoplasma.

For detailed protocol of mycoplasma elimination, Click here: PurMa™ Complete Mycoplasma Removal for Cell Culture

References

  1. Eradication of mycoplasma contaminations. Uphoff et al. Methods Mol Biol. 2013;946:15-26.
  2. The elimination of mycoplasma from infected hybridomas by passaging in BALB/c mice. Carroll et al. J Immunol Methods. 1988 Apr 6;108(1-2):189-93.
Parameter Specification
Appearance Red liquid
pH 7.4 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Indicator NA
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition Dry Ice

 

Additional information

Size

1 ml, 10 ml

Quality Control

[vc-quality-control-tab]

SDS

PurMa™ MTRI, MTRII, MTRIII_SDS

11 reviews for Mycoplasma Treatment Kit (MTK) (100X)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

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