Hybridoma Cryoprotective Media with DMSO (HCM-D)
PurMa™ Hybridoma Cryoprotective Media with DMSO (HCM-D) is specialized for Fused myeloma, CHO cells as well as humanized Hybridomas. Moreover, It contains a proprietary combination for the best hybridoma cryopreservation, survival, and recovery after thawing.
Description
Background
PurMa™ Hybridoma Cryoprotective Media with DMSO (HCM-D) is specialized for Fused myeloma , CHO cells as well as humanized Hybridomas. It contains a proprietary combination for the best hybridoma cryopreservation, survival, and the recovery after thawing.
Why Cryopreservation
During cryopreservation cooling aqueous solutions below the equilibrium freezing point will inevitably result in the formation of ice crystals in the extracellular media. This will results in a decrease in the concentration of water which has two key consequences:
- Osmotic gradient forms across the cell membrane, which causes “cell dehydration”, and
- Ice formation and abnormal concentration of solutes in the intracellular matrix, osmatic shock, and toxicity.
DMSO benefits cryoprotection and cryoprotective agents in several ways
- Provides strong molecular stability.
- Besides, facilitates a high non-conductor constant.
- It contains the basic properties, salts solubility particularly for anions. Additionally contains a propensity to act as the acceptor atom in hydrogen bonding.
- Especially, DMSO makes a hydrogen bond with water which is 30% stronger than between two water molecules.
The proprietary formulation of PurMaTM HCM allows this media to get inside the cells instantly and lower cell death.
Special Features
- Ready-to-Use medium.
- Ready-to-Use.
- PurMaTM Hybridoma Cryoprotective Media (HCM) with DMSO provides much higher cell viability after short/long-term cryoprotection.
- PurMaTM HCM Prevents the formation of crystals in extracellular space. This, consequently, prevents cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.
- Preserves morphological appearance as a standard factor of cell recovery.
- Tested for pH, osmolality, sterility, and endotoxin.
- Each Lot of PurMaTM HCM goes through rigorous testing for pathogens and sterility. We evaluate each Lot as well for several criteria. Including survival (by Trypan Blue method), viability & performance (by MTT Test), and the recovery rate by Alamar Blue test.
- PurMaTM HCM has been tested successfully for several antibody making cell lines including fused NS1 and CHO cells.
- Suitable for clinical applications.
Guideline
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines
References
- The chemical and physical properties of DMSO. MacGregor WS. Ann N Y Acad Sci. 1967 Mar 15;141(1):3-12. doi: 10.1111/j.1749-6632.1967.tb34860.x.
- Improvement of monoclonal antibody production in hybridoma cells by dimethyl sulfoxide. Ling et al. Biotechnol Prog. 2003 Jan-Feb;19(1):158-62. doi: 10.1021/bp020068d.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Size | 1 x 10 ml, 1 x 100 ml |
|---|
Quality Control
[vc-quality-control-tab]11 reviews for Hybridoma Cryoprotective Media with DMSO (HCM-D)
Only logged in customers who have purchased this product may leave a review.
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azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 21-Feb-2025 15:30:00
Ease of placing the order: ‘not given’
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 24-Feb-2025 13:50:14
Ease of placing the order: ‘not given’
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 27-Feb-2025 17:42:44
Ease of placing the order: ‘not given’
Anonymous –
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Time of submission: 05-Mar-2025 16:31:44
Ease of placing the order: ‘not given’
michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 17:29:13
Ease of placing the order: ‘not given’
Anonymous –
Review:
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Time of submission: 10-Mar-2025 14:32:38
Ease of placing the order: ‘not given’
jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5