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PurMa TRIS Buffer Solution

PurMa TRIS Buffer Solution

(11 customer reviews)

PurMa TRIS Buffer Solution is one of the most used buffers in molecular biology, cell culture and protein biochemistry. In fact, it provides a buffering capacity in solutions such as in TAE and TBE buffers. Moreover, PurMa TRIS Buffer Solution has an important impact for nucleic acid applications.

SKU: N/A Category: Cell Culture Suited Buffers Tags: DNase/RNase Free, Calcium and Magnesium Free, Endotoxin Depleted, TRIS Buffer, Nucleic Acid, Cell Culture

Description

Background

PurMa TRIS Buffer Solution is frequently used buffers in molecular biology, cell culture and protein biochemistry. In fact, it provides a buffering capacity  in solutions. Moreover, PurMa TRIS Buffer Solution has an important impact for nucleic acid applications. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g., condensations with aldehydes. The pH of  tris buffer has an important impact on its function .

Grades of Tris Buffer Solutions

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

we finally sterilize the buffers by autoclaving the solution and packing  in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer  through the PurMaTM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B   to an endotoxin-specific absorbent. we finally  eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

Features

  • it has an important role in molecular biology as well as Cell Culture Suited.
  • PurMa Biologics manufactures Tris Buffer from  Mg2+ and Ca 2+-free water.
  • Free of RNAse /DNase
  • Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
  • It is rigorously tested for contamination of endotoxin and mycoplasma.
  • Is manufactured in several pH formats.
  • Sterile filtered by 0.1µm.
  • As it is packed under N2, the pH won’t change over time before opening the bottle.

References

  1. The structure of crystalline tris: a plastic crystal precursor, buffer, and acetylcholine attenuator. Rudman R, Eilerman D, La Placa SJ. Science. 1978 May 5;200(4341):531-3. doi:10.1126/ science. 644311. PMID: 644311.
  2. Interactions of tris buffer with nucleotides: the crystal structure of tris(hydroxymethyl) methylammonium adenosine 5′-diphosphate dihydrate. Shakked Z, Viswamitra MA, Kennard O. Biochemistry. 1980 Jun 10;19(12):2567-71. doi: 10.1021/bi00553a005. PMID: 7397089.
Parameter Specification
Appearance Clear liquid
pH  7.4 ± 0.1; 8.0 ± 0.1; 9.0 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  N/A
Indicator  N/A
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Concentration

0.1 M, 0.5 M, 1 M, 2 M
Grade

Regular, RNase and DNase Free, Cell Culture Suited, Ca and Mg Free, Cell Culture Suited, Ca and Mg Free, Endotoxin Depleted
pH

pH 4.0, pH 5.0, pH 6.0, pH 6.5, pH 6.8, pH 7.2, pH 7.4, pH 8.0, pH 9.0, pH 11.0
Size

1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml

11 reviews for PurMa TRIS Buffer Solution

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

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