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Trypsin-EDTA 0.5%

Trypsin-EDTA 0.5%

(11 customer reviews)

PurMa™ Trypsin-EDTA 0.5% contains ultra-pure trypsin. Furthermore, trypsin is one of the members of the serine protease family. Cell culture laboratories widely use trypsin to dissociate the adherent mammalian cells for routine cell culture. The Trypsin family is conservative. Trypsin performs the best at 37oC and at the PH 7.3-8.5.

SKU: N/A Categories: Trypsin With EDTA, Trypsin EDTA and EDTA-Free Tags: Trypsin, Trypsin with EDTA 0.5%

Description

Background

PurMa™ Trypsin-EDTA 0.5% contains ultra-pure trypsin. Furthermore, trypsin is one of the members of the serine protease family which goes through a set of intracellular signaling results in the activation of this enzyme in pancreas. Cell culture laboratories widely use trypsin to dissociate the adherent cells for routine cell culture. The Trypsin family is conservative. Trypsin performs the best at 37oC and at the PH 7.3-8.5.

Trypsin Activity

Trypsin cleaves its substrates: IGDKLIPA unless these amino acids are followed by proline. PurMa™ Trypsin is HPLC purified 99% purity for bovine pancreas. Moreover, optical rotatory dispersion of trypsin in the presence of calcium shows a major conformational transition between 40 and 45° which results in deactivation of trypsin. Therefore, all the buffers needed to be Ca+2 & Mg+2 free for the proper function of this enzyme.

PurMa™ Trypsin Deactivates Mycoplasma in your Cell Culture

Trypsin treatment abolishes the ability of mycoplasmas to attach to the cells. Mycoplasma is one of the common and slowly deteriorating contamination in cell culture, especially due to the invisibility of the contamination. PurMa Biologics manufactures all the reagents you need to fight mycoplasma in your cell culture. The presence of a trace of SDS and other detergents leads to a decrease in the trypsin activity.

Preparation

The activity of this enzyme is further modulated by the sulfation of Tyr154. To overcome the mentioned issue, we use our most sophisticated water quality to ensure the absence of Mg+2 and Ca+2 as well as other heavy metals, please see click here: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

Additionally, we include 2mM EDTA as it Chelates metals such as calcium, magnesium, lead, mercury, copper, iron…

Features and Benefits

  • PurMa™ Trypsin does not contain calcium or magnesium ions or any other heavy metals.
  • PurMa™ Trypsin does not contain serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF.
  • PurMa™ Trypsin does not contain Phytates or salts of phytic acid, such as myo-inositol which is commonly present in cell culture media and inhibits trypsin activity.
  • PurMa™ Trypsin has been rigorously evaluated for yeast, mold, mycoplasma, and endotoxin contamination.

Concentrations

PurMa Biologics manufactures 0.05%, 0.25%,0.5%, 2.5% and 5% Trypsin with EDTA as well as without EDTA in phenol red or without phenol red. Moreover, PurMa™ Trypsin is made in Hanks’ Buffer with 2mM EDTA (pH 7.8).

Formulation

The complete formulation can be found here: 0.5% Trypsin with EDTA 

References

  1. Fate of trypsin and chymotrypsin in the human small intestine. Goldberg et al. 1969 Jun;10(6):477-83. doi: 10.1136/gut.10.6.477. PMID: 5786145.
  2. Adhesion of mycoplasmas to eukaryotic cells. Razin et al. Ciba Found Symp. 1981;80:98-118. doi: 10.1002/9780470720639.ch8. PMID: 6790254.
  3. Effects of hyaluronidase, trypsin, and EDTA on surface composition and topography during detachment of cells in culture. Vogel KG. Exp Cell Res. 1978 May;113(2):345-57. doi: 10.1016/0014-4827(78)90375-0. PMID: 122554.
Parameter Specification
Appearance Red, clear liquid
pH  7.8 ± 0.1
Osmolality  275-360 mOsm/L
Endotoxin  NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive  EDTA
Indicator  Phenol red
Mycoplasma Detection Negative
Sterility Tested  Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

Trypsin 0.5%, Standard Formulation, Trypsin 0.5%, Without Sodium Bicarbonate, Trypsin 0.5%, Without Calcium Chloride, Trypsin 0.5%, Without Glucose
Size

1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml
Phenol Red

With Phenol Red, Without Phenol Red

Quality Control

[vc-quality-control-tab]

Formulation

0.5% Trypsin-EDTA Formulations

SDS

SDS

11 reviews for Trypsin-EDTA 0.5%

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

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