Ultra-Premium Fetal Horse Serum (UP-FHS)
Reproducibility and consistency regarding In vitro cell culture experiments is a matter of concern. The inconsistency in In vitro cell culture is related to batch-wise variations of biological materials, mainly the serum content. PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS) has far fewer fluctuations compared to other serums in the market. That is due to the vigorous analysis of this serum and, more importantly, the age of the fetus.
Description
Background
Reproducibility and consistency regarding In vitro cell culture experiments is a matter of concern. The inconsistency in In vitro cell culture is related to batch-wise variations of biological materials, mainly the serum content. PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS) has far less fluctuations compared to other serums in the market. That is due to the vigorous analysis of this serum and, more importantly, the age of the fetus.
PurMa Biologics has three main premium fetal horse serums
- PurMa™ Premium Fetal Horse Serum (P-FHS).
- PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS).
- PurMa™ Endotoxin Depleted Fetal Horse Serum (EDP-FHS).
The Major Preference of UP-FHS Over P-FHS
- The source of the Blood and therefore:
- Moreover, the significant higher growth by UP-FBS compared to P-FHS
- Also, the depth of the analysis
- Besides, the parameters are evaluated. This helps with the consistency of analysis using various Lots.
PurMa Biologics routinely tests PurMa™Ultra-Premium Fetal Horse Serum (UP-FHS) for the following elements. That is of course, besides the routine tests which is provided in the “Serum Analysis Tab “.
Routine Quality Analysis
With relative order of importance: IgG, Total Protein, Iron, Percent Iron Saturation, Inorganic Phosphorus, Glucose, Alkaline Phosphatase, Potassium, Albumin, Blood Urea Nitrogen, Sodium, Calcium, Chloride, Total Bilirubin, Creatinine, Gamma Globulin, Lactate Dehydrogenase, TIBC, SGOT, SGPT.
PurMa™ Ultra-Premium FHS
- All three PurMa™ Ultra-Premium FHS are, indeed, of USA origin.
- We additionally sterilize FHS by gamma irradiation.
- Have been heat inactivated.
- PurMa Biologics vigorously analyzes all bovine and horse serums and, that results in a certificate of analysis for each Lot.
Routine pathological analysis on PurMa bovine and horse serums: Routine Pathological Analysis on PurMa Bovine and Horse Serums
References
- Higher percentage of horse serum in culture media blocks attachment of PC12 cells. Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.
- Changes in serum influence the fatty acid composition of established cell lines. Stoll et al. In Vitro. 1984 Sep;20(9):732-8.
Methodology | Unit measured | Accepted level | Result |
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
pH | N/A | measured | 6.58 |
Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
IgG | Elliza | Measured | 0.19 ug/ml |
Albumin | Eliza | Measured | ≤ 4.6 g/dL |
Virus Testing | |||
Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
Horse West Nile Virus | NA | Not Detected | Not Detected |
Rabies | NA | Not Detected | Not Detected |
Reovirus | NA | Not Detected | Not Detected |
Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
Bluetongue | NA | Not Detected | Not Detected |
Additional information
Size | 1 x 50 ml, 1 x 500 ml |
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11 reviews for Ultra-Premium Fetal Horse Serum (UP-FHS)
Only logged in customers who have purchased this product may leave a review.
azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 21-Feb-2025 15:30:00
Ease of placing the order: ‘not given’
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 24-Feb-2025 13:50:14
Ease of placing the order: ‘not given’
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 27-Feb-2025 17:42:44
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 16:31:44
Ease of placing the order: ‘not given’
michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 17:29:13
Ease of placing the order: ‘not given’
Anonymous –
Review:
Have you purchased:
Time of submission: 10-Mar-2025 14:32:38
Ease of placing the order: ‘not given’
jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5