PurMa™ Cell Culture Media contains commonly used, well described media for growing various primary as well as commercial cell lines.
Our Cell Culture line includes:
- DMEM (Dulbecco’s Modified Eagle’s Medium)
- DMEM/F-12 (Dulbecco’s Modified Eagle’s Medium with Nutrient Mixture F-12)
- HAM’S F-12K (Kaighn’s Modification of Ham’s F-2 Medium)
- RPMI 16400 (Roswell Park Memorial Institute 1640)
- IMDM (Iscove’s Modified Dulbecco’s Medium)
- EMEM (Eagle’s Minimal Essential Medium)
- McCoy’s 5A Modified Media
- Williams’ E Medium
- Leibovitz’s L-15 Medium
- GMEM (Glasgow’s Modified Eagle’s Medium – Glutamine Free)
- MEM (Minimum Essential Media)
- MEM-α (Minimum Essential Media Alpha)
- MEM-α-N (Minimum Essential Media Alpha with Nucleosides)
- BME (Basal Medium Eagle).
PurMa™ MEM, MEM-α, MEM-α-N, and BME cell culture medias are available in either EBSS (Earle’s Balanced Salt Solution) or HBSS (Hanks’ Balanced Salt Solution).
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Dulbecco’s Modified Eagle’s Medium (DMEM) (3)
Dulbecco’s Modified Eagle’s Medium (DMEM) is a modification of Basal Medium Eagle. Our DMEM, optimized to deliver exceptional growth and consistency, is available in Standard, MAX, and Advanced formulations. Dulbecco’s Modified Eagle’s Medium (DMEM) contains fourfold more amino acids and vitamins compared to the original formulation. DMEM is a good choice for growing the primary cell culture of mouse and… -
Basal Medium Eagle (BME) in Earle’s Buffer (4)
Basal Medium Eagle (BME) in Earle’s Buffer Background Basal Medium Eagle (BME) in Earle’s Buffer was originally developed by Harry Eagle. this media is one of the most widely used of all synthetic cell culture media. Furthermore, BME, when properly supplemented, has demonstrated wide applicability, for supporting monolayer growth of a wide variety of normal and transformed cell lines. Moreover,… -
DMEM F-12 (2)
DMEM-F-12 (Dulbecco’s Modified Eagle Medium Nutrient-Mixture F-12) provides superior quality, consistency, and great control in mammalian cell culture. This formulation combines DMEM’s high concentrations of glucose, amino acids, and vitamins as well as much more variation of salts with DMEM-F-12 ’s wide variety of components. This media is better than DMEM and advanced IMDM on MDCK, glial cells, fibroblasts, human… -
Basal Medium Eagle (BME) in Hanks’ Buffer (3)
asal Medium Eagle (BME) in Hanks’ Buffer Background Basal Medium Eagle (BME) in Hanks’ Buffer originally developed by Harry Eagle. Moreover, this media is one of the most widely used of all synthetic cell culture media. Markedly, BME is the predecessor of Eagle’s Minimum Essential Medium (MEM) and Dulbecco’s Modified Eagle’s Medium (DME). PurMa Biologics presently manufactures BME with Earle’s… -
Kaighn's Modification of Ham's F-12K Medium (4)
Kaighn's Modification of Ham's F-12K Medium is a modified version of Ham’s F-12 medium. Ham’s F-12K (Kaighn’s) worked properly for primary cells such as human oocytes and hepatocytes. This medium also performed well for murine and chicken liver cells. it contains a variety of components not found in older media. That includes putrescine thymidine, hypoxanthine, zinc, and higher levels of… -
Roswell-Park Memorial Institute 1640 (3)
Roswell Park Memorial Institute 1640 (RPMI-1640) is commonly used for Mammalian cell culture. RPMI-1640 contains higher PH, biotin, vitamin B12, and PABA. PurMa Biologics manufactures Standard, Max and Advanced formulations of RPMI-1640 for diverse cell culture research requirements. Furthermore, we offer a variety of conditions and sizes of RPMI-1640. Roswell Park Memorial Institute 1640 (RPMI-1640) was originally developed in 1967… -
Iscove's Modified Dulbecco's Medium (IMDM) (3)
Although Iscove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating cell lines, it is certainly quite suitable for slow-growing cells as well. Moreover, this medium is the only medium that lacks Iron, with potassium nitrate replacing ferric nitrate, therefore making it an excellent choice for adhesive cells. scove’s Modified Dulbecco’s Medium (IMDM) is specialized for rapidly proliferating, high-density cell… -
Eagle's Minimal Essential Medium (EMEM) (3)
Eagle’s Minimum Essential Medium (EMEM) Background Eagle’s Minimal Essential Medium (EMEM) was developed by Harry Eagle and is widely used. It is composed of necessary salts for optimal cell growth and maintenance. Additionally, it contains high glucose, essential amino acids, and vitamins. Several versions of EMEM have been developed by modification of its composition. This media is suitable for a… -
McCoy’s 5A Modified Media (M5AMM) (3)
McCoy’s 5A Modified Media (M5AMM) Background McCoy’s 5A Modified Media (M5AMM) was originally used for Walker Carcinosarcoma 256 Cells. It was subsequently modified to create a new medium known as McCoy’s 5A. Additionally, M5AMM contains several folds of amino acids and vitamins compared to the original formulation. moreover, our McCoy’s 5A covers the main needs for your cell lines, delivers… -
William's E Medium (3)
Williams′ Medium E Background Williams′ Medium E is a modification version of Williams′ Medium. Although it is like MEM, there is differences in glucose as well as amino acid contents. This media was originally used to isolate and culture primary liver epithelial cells from Epithelial-like cells of fibroblast-like cells culture. It has been shown that it is suited for longer-term… -
Leibovitz’s L-15 Medium (2)
Leibovitz’s L-15 Medium Background Leibovitz’s L-15 Medium was originally designed to be used in carbon dioxide (CO2) free systems. Therefore, does not need sodium bicarbonate to provide the buffering capacity. Instead, phosphates, free base amino acids, salts, and galactose maintain the physiological pH. This media contains: High Level of Sodium pyruvate (5mM) Undoubtedly, the absence of Sodium Bicarbonate makes this… -
Glasgow’s Modified Eagle’s Medium (3)
Ian McPherson and Michael Stoker developed Glasgow’s Modified Eagle’s Medium. they used polyoma virus to transform four fibroblast clones from a culture of baby hamster kidney cells. As a result of the Particular criteria mentioned below this media is used without serum. Needs to be supplemented with 10% Tryptose phosphate broth. Moreover, it contains twice the amount of amino acids… -
Minimum Essential Media (MEM) in Earle’s Buffer (4)
Minimum Essential Media (MEM) in Earle’s Buffer Background Minimum Essential Media (MEM) in Earle’s Buffer is a modification of Basal Medium Eagle (BME) which was originally developed by Harry Eagle. It also includes higher concentrations of amino acids for cultured mammalian cells. Moreover, MEM is available with Earle’s salts for use in a CO2 incubator, or with Hanks’ salts for… -
MEM Alpha Modification in Earle’s Buffer (MEM-α) (3)
MEM Alpha Modification in Earle’s Buffer (MEM-α) Background MEM Alpha Modification in Earle’s Buffer (MEM-α) certainly suits mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM -α Modification applies for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood. Eliminating calcium in this media facilitates the growth of cells in suspension… -
Minimum Essential Media (MEM) In Hanks’ Buffer (3)
Minimum Essential Media (MEM) In Hanks’ Buffer Background Minimum Essential Media (MEM) In Hanks’ Buffer is a modification of Basal Medium Eagle . It was originally developed by Harry Eagle for specific requirements of certain subtypes. Additionally, it includes higher concentrations of amino acids which is more suitable for cultured mammalian cells. Moreover, PurMa Biologics manufactures MEM with Earle’s buffer… -
MEM Alpha Modification (MEM-α) in Hanks’ Buffer (3)
MEM Alpha Modification (MEM-α) in Hanks’ Buffer Background MEM Alpha Modification (MEM-α) in Hanks’ Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. Besides, this media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells. Furthermore, it is important… -
MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) (3)
MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) Background MEM Alpha with Nucleosides in Earle’s Buffer (MEM-α-N-EBSS) is a suitable nutrient for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media contains nucleoside, and the formulation is available below. depending on cell types, laboratories use MEM-α-N for growing mammalian cell for a variety of suspension as… -
MEM α-Modification with Nucleosides in Hanks’ Buffer (4)
MEM α-Modification with Nucleosides in Hanks’ Buffer Background MEM α-Modification with Nucleosides in Hanks’ buffer provides a great nutritional culture environment during mammalian cells. This media, as well plays a key role in the process of selection of transfected DHFR-negative cells. Moreover, MEM-α-N-HBSS is widely used for a variety of suspension and adherent cells. Calcium, however, plays a crucial role…