Kaighn’s Modification of Ham’s F-12K Medium is a modified version of Ham’s F-12 medium. Ham’s F-12K (Kaighn’s)  worked properly for primary cells such as human oocytes and hepatocytes. This medium also performed well for murine and chicken liver cells.
it contains a variety of components not found in older media. That includes putrescine thymidine, hypoxanthine, zinc, and higher levels of all amino acids and sodium pyruvate. These compensated for supplemental serum. This media still needs the addition of 10 % FBS . this medium  Medium contains no proteins or growth factors and therefore needs FBS. F-12K Medium also contains: Low level of glucose (1.26 g/ liter), Sodium bicarbonate (2.5 g/L) (if
PurMa Biologics also offers F-12K Medium with low sodium bicarbonate (1.5 g/L) (Cat # P3S057108) as well as high sodium bicarbonate (3.7g/L) (Cat # P3S032108)
Kaighn’s Modification of Ham’s F-12K Medium also contains 2 mM L-glutamine (292 mg/L)’, A higher concentration of amino acids, vitamins, inositol and choline are present in this medium.
PurMa Biologics manufactures 96 types of Ham’s F-12K which accommodate any form of this medium.

  • Advanced Kaighn’s Modification of Ham’s F-12 (A-Ham’s F-12K) Quick View
  • Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) Quick View
  • Kaighn’s Modification of Ham’s F-12-K Medium Quick View
  • Kaighn’s Modification of Ham’s F-12-MAX (Ham’s F-12K-MAX) Quick View