Roswell Park Memorial Institute 1640 (RPMI-1640) is commonly used for Mammalian cell culture. RPMI-1640 contains higher PH, biotin, vitamin B12, and PABA.

PurMa Biologics manufactures Standard, Max and Advanced formulations of RPMI-1640 for diverse cell culture research requirements. Furthermore, we offer a variety of conditions and sizes of RPMI-1640.

Roswell Park Memorial Institute 1640 (RPMI-1640) was originally developed in 1967 by Moore et. Al at Roswell Park Memorial Institute. Therefore, it has the acronym RPMI-1640. Moreover, this medium contains a great deal of phosphate which is an essential element for tumor cells due to using glycolysis as a main source of energy in cell aggregates which is one of the most useful strategies for tumor cells survival.

RPMI-1640 media are successfully used for:

  • culturing human normal and neoplastic leukocytes
  • HeLa,
  • Jurkat,
  • MCF-7
  • PC12
  • PBMC
  • astrocytes
  • carcinomas
  • bone marrow
  • and hybridoma cells

The major difference between RPMI1640 and other media, besides higher PH (8), is that it contains biotin, vitamin B12, and PABA. In addition to alterations in the amounts of amino acids and vitamins, inositol and choline are present in very high concentrations.

  • Advanced Roswell-Park Memorial Institute 1640 (A-RPMI-1640) Quick View
  • Roswell Park Memorial Institute 1640 (RPMI-1640) Quick View
  • Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX) Quick View