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Taq Polymerase is a thermostable DNA Polymerase enzyme with significant processivity that is most commonly used in PCR. The recombinant
Taq Polymerase is a thermostable DNA Polymerase enzyme with significant processivity that is most commonly used in PCR. The recombinant Taq DNA Polymerase is cloned and expressed in E. coli that shows identical characteristics to native Taq from the thermophilic eubacterium Thermus aquaticus in all aspects of PCR performance. Its terminal transferase activity results in addition of a single nucleotide (adenosine) at the 3' end of the extension product. Taq DNA Polymerase catalyzes the incorporation of dNTPs into DNA in the presence of a DNA template, primer terminus and a cofactor (Magnesium). Taq DNA Polymerase contains a polymerization dependent 5′-3′ exonuclease activity which makes DNA products with A (adenine) overhangs at their 3' end that is important in ligation of the PCR product into the plasmid vector. Despite of having no proof-reading function (as It lacks 3′-5′ exonuclease activity), the enzyme is fully capable to synthesize DNA in vitro with reasonable fidelity. It shows no tendency to mis incorporate nucleotides even after repeated use for cycle sequencing. Recombinant Taq enzyme has a molecular weight of 95-kDa and shows optimal 5’-3’ polymerase activity between 75-80 °C with a half-life of 40 minutes at 95 °C. It can replicate a DNA strand of 1000 bp in less than 10 seconds at 72 °C. Specifications: PurMaTM Taq polymerase is a proprietary formulation of Taq DNA polymerase with the following specifications: *High Fidelity *High thermostability * DNA amplification with High sensitivity *Produced and purified from recombinant E. coli that contains DNA Polymerase gene of Thermus aquaticus * Robust DNA amplification with Higher processivity *High Purity with strictly quality controlled *Amplification of PCR products up to 5 kb in size Composition: Our Taq DNA Polymerase comes with the following components in separate tubes: * PurMaTM Taq * 10X PCR buffer (contains: 500 mM Tris-Cl pH 9.2,160 mM ammonium sulfate, 0.25% Brij 58, and 35 mM magnesium chloride hexahydrate). * Nuclease free H20. Application of PurMaTM Taq:
- PCR (for simple and complex genomic, viral, and plasmid templates)
- Other primer-extension reactions, such as cycle sequencing, sequencing ssDNA
- DNA labeling
Taq Polymerase is a thermostable DNA Polymerase enzyme with significant processivity that is most commonly used in PCR.