Advanced Eagle’s Minimal Essential Medium (A-EMEM)
Advanced Eagle’s Minimal Essential Medium (A-EMEM) contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, A-EMEM needs 2-5% FBS and provides more consistency.
Description
Background
PurMaTM Advanced Eagle’s Minimal Essential Medium (A-EMEM) contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-EMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.
Contents
Advanced EMEM Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-EMEM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Eagle’s Minimal Essential Medium (A-EMEM) is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced EMEM (CD-A-EMEM) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced EMEM Formultaion
References
- Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, Dixon OW, Lizzio EF. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
- Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, Sedwick WD, Brown OE, Laszlo J. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A-EMEM Standard Formulation, A-EMEM w/o L-Alanyl-L-Glutamine, Advanced EMEM w/o Phenol Red, A-EMEM w/o Sodium Bicarbonate, A-EMEM w/o Sodium Pyruvate, A-EMEM w/o Glucose, A-EMEM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, A-EMEM w/o Phenol Red, w/o Sodium Bicarbonate, A-EMEM w/o Sodium Bicarbonate, w/o Sodium Pyruvate, A-EMEM w/o L-Alanyl-L-Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, A-EMEM High Glucose (4.5 g/L), A-EMEM High Sodium Bicarbonate (2.438 g/L), A-EMEM High Glucose (4.5 g/L), High Sodium Bicarbonate (2.438 g/L), A-EMEM 15 mM HEPES (3.6 g/L), Advanced EMEM 25 mM HEPES (5.9 g/L), Advanced EMEM w/o Sodium Pyruvate, w/o Phenol Red, A-EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), A-EMEM w/o Phenol Red, with 25 mM HEPES (5.9 g/L), A-EMEM w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
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