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Advanced Glasgow’s Modified Eagle’s Medium – PurMa Biologics LLC

Advanced Glasgow’s Modified Eagle’s Medium is free of glutamine contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction.  Additionally, Advanced Glasgow’s medium needs 2-5% FBS and provides more consistency.

Description

Background

PurMaTM Advanced Glasgow’s Modified Eagle’s Medium is free of glutamine amino acids. Moreover, it contains components that allow for a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increase consistency. This results in less variation amongst lot-to-lot serum changes. In A-GMEM, L-Alanyl-L-Glutamine is used as the source of glutamine. Additionally, reducing FBS requirements lowers the cost of cell culture experiments.

Contents

Advanced Glasgow’s Contains:

  • includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
  • A-GMEM incorporates elements that significantly reduce the need for a serum for cell culture.

Crucial Ingredients:

  • PurMaTM Human Transferrin (Holo): prevents Improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
  • PurMaTM Insulin Recombinant
  • Trace of other elements which mimic the elements in fetal bovine serum.

Consequently, Advanced Glasgow’s Modified Eagle’s Medium  is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined  Glasgow’s medium  (CD-Glasgow’s meium) is the outcome of decades of cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.

Formulation

Complete formulation is available here: Advanced Glasgow’s Formulation 

References

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

A- GMEM Standard Formulation, A- GMEM w/o Phenol Red, A- GMEM w/o Sodium Bicarbonate, A- GMEM w/o Sodium Pyruvate, A- GMEM w/o Glucose, A- GMEM Low Glucose (1.5 g/L), A- GMEM Low Sodium Bicarbonate (1.1 g/L), A- GMEM 15 mM HEPES (3.6 g/L), A- GMEM 25 mM HEPES (5.9 g/L), A- GMEM 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.1 g/L), A- GMEM 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.1 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

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