Advanced Minimum Essential Media (A-MEM) in EBSS
Advanced Minimum Essential Media (A-MEM) in Earle’s Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced MEM needs 2-5% FBS and provides more consistency.
Description
Background
PurMaTM Advanced Minimum Essential Media (A-MEM) in Earle’s Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. Consequently, this results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in A-MEM. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.
Contents
Advanced MEM in EBBS Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-MEM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Minimum Essential Media (A-MEM) in Earle’s Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM (CD-A-MEM) is the outcome of decades of innovative cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced MEM Formulation in Earle’s Buffer
References
- Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie JA, Byard R, Dardick I, Tsang BK. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
- Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
- Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | A- MEM Standard Formulation, A- MEM w/o L-Alanyl-L-Glutamine, A- MEM w/o Phenol Red, A- MEM w/o Sodium Bicarbonate, A- MEM w/o Glucose, A- MEM w/o L-Alanyl-L-Glutamine , w/o Phenol Red, A- MEM w/o L-Alanyl-L-Glutamine , w/o Sodium Bicarbonate, A- MEM w/o L-Alanyl-L-Glutamine , w/o Phenol Red, w/o Sodium Bicarbonate, A- MEM 15 mM HEPES (3.6 g/L), A- MEM 25 mM HEPES (5.9 g/L), A- MEM With Sodium Pyruvate (0.11 g/L), A- MEM High Glucose (4.5 g/L), A- MEM High Sodium Bicarbonate (3.7 g/L), A- MEM Low Sodium Bicarbonate (0.35 g/L), A- MEM Low Sodium Bicarbonate (0.85 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
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