Advanced Minimum Essential Media (A-MEM) in HBSS
Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer contains L-Alanyl-L-Glutamine as well as ingredients to allow for serum reduction. Additionally, Advanced MEM needs 2-5% FBS and provides more consistency.
Description
Background
PurMaTM Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer contains components to provide a 60–90% reduction in FBS supplementation. More importantly, using synthetic components such as recombinant insulin, albumin, and transferrin increases consistency. Consequently, this results in less variation amongst lot-to-lot serum changes. L-alanyl-l-glutamine is the source of glutamine in A-MEM. Additionally, reducing the requirement for FBS lowers the cost of cell culture experiments.
Contents
Advanced MEM in HBSS Contains:
- includes all twenty essential and non-essential amino acids with higher concentrations than regular and MAX formulations.
- A-MEM incorporates elements that significantly reduce the need for a serum for cell culture.
Crucial Ingredients:
- PurMaTM Human Transferrin (Holo): prevents improper iron storage and delivery in cell culture systems which is a major contributor to oxidative stress and protein damage.
- PurMaTM Insulin Recombinant
- Trace of other elements which mimic the elements in fetal bovine serum.
Consequently, Advanced Minimum Essential Media (A-MEM) in Hanks’ Buffer is a significant step toward a chemically defined form of this media. Unquestionably, Chemically Defined Advanced MEM (CD-A-MEM) is the outcome of decades of innovative cutting-edge research and will soon be offered globally to laboratories that are looking for consistency and repeatability in their data.
Formulation
Complete formulation is available here: Advanced MEM (HBSS) Formulation in Hanks’ Buffer
References
- Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
- Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846
Parameter | Specification |
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Appearance | Red, clear liquid |
pH | 7.2 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | Sodium pyruvate |
Indicator | Phenol red |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Additional information
Condition | A-MEM (HBSS) Standard Formulation, A-MEM (HBSS) w/o L-Alanyl-L-Glutamine, A-MEM (HBSS) w/o Phenol Red, A-MEM (HBSS) w/o Sodium Bicarbonate, A-MEM (HBSS) w/o Glucose, A-MEM (HBSS) w/o L-Alanyl-L-Glutamine , w/o Phenol Red, A-MEM (HBSS) w/o L-Alanyl-L-Glutamine , w/o Sodium Bicarbonate, A-MEM (HBSS) w/o L-Alanyl-L-Glutamine , w/o Phenol Red, w/o Sodium Bicarbonate, A-MEM (HBSS) 15 mM HEPES (3.6 g/L), A-MEM (HBSS) 25 mM HEPES (5.9 g/L), A-MEM (HBSS) With Sodium Pyruvate (0.11 g/L), A-MEM (HBSS) High Sodium Bicarbonate (0.85 g/L), A-MEM (HBSS) High Glucose (4.5 g/L), A-MEM (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), A-MEM (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L) |
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Format | Liquid, Powder |
Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
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