Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer
Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. BME-MAX in HBBS significantly improves cell viability and growth. We manufacture several types of BME-MAX based on your requirements.
Description
Background
Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer is a commonly used media for Mammalian cell culture.
Advantages
The advantages of BME-MAX over BME are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.
In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.
Contents
Basal Medium Eagle-MAX (BME-MAX) in Hanks’ Buffer contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of BME-MAX in HBBS based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
BME-MAX in HBBS contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).
Formulation
The complete formulation can be found here: BME-MAX with Hanks’ Buffer (HBSS) Formulation
References
- Improved chemically defined basal medium (CMRL-1969) for primary monkey kidney and human diploid cells. Healy GM, et al. Appl Microbiol. 1971 Jan;21(1):1-5. doi: 10.1128/am.21.1.1-5.1971. PMID:
- Nutritional requirements for the production of herpes simplex virus. I. Influence of glucose and glutamine of herpes simplex virus production by HeLa cells. LEWIS VJ Jr, SCOTT LV. J Bacteriol. 1962 Mar;83(3):475-82. doi: 10.1128/jb.83.3.475-482.1962. PMID: 14464909
- An improved culture system for secondary palatal elevation. Lewis CA, Thibault L, Pratt RM, Brinkley LL. In Vitro. 1980 Jun;16(6):453-60. doi: 10.1007/BF02626457. PMID: 7390537
Parameter | Specification |
---|---|
Appearance | Red, clear liquid |
pH | 7.2 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | N/A |
Indicator | Phenol red |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Additional information
Condition | BME-MAX (HBSS) Standard Formulation, BME-MAX (HBSS) w/o Phenol Red, BME-MAX (HBSS) w/o Sodium Bicarbonate, BME-MAX (HBSS) w/o Glucose, BME-MAX (HBSS) 15 mM HEPES (3.6 g/L), BME-MAX (HBSS) 25 mM HEPES (5.9 g/L), BME-MAX (HBSS) With Sodium Pyruvate (0.11 g/L), BME-MAX (HBSS) High Sodium Bicarbonate (0.85 g/L), BME-MAX (HBSS) High Glucose (4.5 g/L), BME-MAX (HBSS) 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (0.85 g/L), BME-MAX (HBSS) 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (0.85 g/L) |
---|---|
Format | Liquid, Powder |
Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
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