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DMEM F12 W500-BG_PNG

Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)

(11 customer reviews)

Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) is commonly used worldwide. Moreover, DMEM F-12 contains more nutrition and offers more buffering capacity. Therefore, this medium provides more growth and maintenance ability.

SKU: N/A Categories: Kaighn's Modification of Ham's F-12K Medium, Cell Culture Media Tags: Ham's F-12K, Kaighn's, Ham's F-12

Description

Background

Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12) provides superior quality, consistency, and great control in mammalian cell culture. DMEM F-12 is preferred for MDCK, glial cells, fibroblasts, human endothelial cells, as well as rat fibroblasts. In general, DMEM: F-12 is more enriched but contains the following major changes compared to DMEM:

Ingredients

  • It contains 2.5 mM L-glutamine
  • 0.5 mM sodium pyruvate
  • Moreover, contains 30mM NaHCO3 (2.438g/L)
  • High glucose
  • Non-Essential Amino Acids
  • also, contains Sodium pyruvate
  • Phenol Red
  • L-glutamine

 Formulation

For complete formulation click here: DMEM F-12 Formultaion

References:

  1.  Clonal growth of mammalian cells in a chemically defined, synthetic medium . DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016 /0042-68 22(59)90043-1. PMID: 13669362.
  2. Plaque production by the polyoma virus. DULBECCO et al. 1959 Jul;8(3):396-7. doi: 10.1016/0042-6822(59)90043-1. PMID: 1366936.

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

DMEM F-12 Standard Formulation, DMEM F-12 Standard Formulation, ATCC Formulation, DMEM F-12 w/o Glutamine, DMEM F-12 w/o Phenol Red, DMEM F-12 w/o Sodium Bicarbonate, DMEM F-12 w/o Sodium Pyruvate, DMEM F-12 w/o Glucose, DMEM F-12 w/o Lipoic Acid, DMEM F-12 w/o Glutamine, w/o Phenol Red, DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, DMEM F-12 w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, DMEM F-12 w/o Phenol Red, w/o Sodium Bicarbonate, w/o Sodium Pyruvate, DMEM F-12 Low Glucose (1.5 g/L), DMEM F-12 Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 15 mM HEPES (3.6 g/L), DMEM F-12 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 w/o Sodium Pyruvate, w/o phenol Red, DMEM F-12 w/o Phenol Red, with 15 mM HEPES (3.6 g/L), DMEM F-12 w/o Phenol Red, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 w/o Sodium Pyruvate, Low Sodium Bicarbonate (1.2 g/L), DMEM F-12 High Glucose (4.5 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

11 reviews for Dulbecco’s Modified Eagle’s Medium/F-12 (DMEM F-12)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

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