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Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX)

Dulbecco’s Modified Eagle’s Medium-MAX (DMEM-MAX)

(11 customer reviews)

Dulbecco′s Modified Eagle′s Medium MAX (DMEM-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life.  DMEM-MAX significantly improves cell viability and growth. We manufacture several types of DMEM-MAX based on your requirements.

SKU: N/A Categories: Dulbecco’s Modified Eagle’s Medium (DMEM), Cell Culture Media Tags: Without Phenol Red, Mammalian Cells, Cell Culture, DMEM, MAX, L-Alanyl-L-Glutamine, glucose, Phenol Red

Description

Background:

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) is a commonly used media for Mammalian cell culture.

Advantages:

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) over DMEM:

Higher PH Capacity

Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:

Possess longer shelf life.

More importantly, minimizes toxic ammonia build-up.

Significantly improves cell viability and growth.

Remains stable across a wide range of temperatures.

Improves the culture of primary cells.

Mechanism:

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents:

Dulbecco′s Modified Eagle′s Medium-MAX (DMEM-MAX) contains:

  •     5 mM sodium pyruvate
  •     High glucose (4.5g/L)
  •     Non-Essential Amino Acids
  •     3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of DMEM -MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  •     Phenol Red

DMEM-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation:

For complete formulation Click here:  DMEM MAX Formulation

References:

  1. A cell culture medium that supports the differentiation of human retinal pigment epithelium into functionally polarized monolayers. Hu J, Bok D. Mol Vis. 2001 Feb 7;7:14-9. PMID: 11182021
  2. Induction of differentiation by pyruvate and DMEM in the human retinal pigment epithelium cell line ARPE-19. Ahmado A, et al., Invest Ophthalmol Vis Sci. 2011 Sep 9;52(10):7148-59. doi: 10.1167/iovs.10-6374.PMID: 21743014
  3. Protective layer formation on magnesium in cell culture medium. Wagener V, et al., Mater Sci Eng C Mater Biol Appl. 2016 Jun;63:341-51. doi: 10.1016/j.msec.2016.03.003. Epub 2016 Mar 3.PMID: 27040228
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

DMEM-MAX- Standard Formulation, DMEM-MAX- Standard Formulation, ATCC Formulation, DMEM-MAX- Without Phenol Red, DMEM-MAX- Without Sodium Bicarbonate, DMEM-MAX- With Sodium Pyruvate, DMEM-MAX- Without Glucose, DMEM-MAX- With Lipoic Acid, DMEM-MAX- Without Glutamine, Without Phenol Red, DMEM-MAX- Without Glutamine, Without Sodium Bicarbonate, DMEM-MAX- Without Glutamine, Without Phenol Red, Without Sodium Bicarbonate, DMEM-MAX- Low Glucose (1.5 g/L), DMEM-MAX- Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX- 15 mM HEPES (3.6 g/L), DMEM-MAX- 25 mM HEPES (5.9 g/L), DMEM-MAX- 15 mM HEPES (3.6 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX- 25 mM HEPES (5.9 g/L), Low Sodium Bicarbonate (1.5 g/L), DMEM-MAX- Without Phenol Red, Without Sodium Bicarbonate, DMEM-MAX- Without phenol Red, 15 mM HEPES (3.6 g/L), DMEM-MAX- Without phenol Red, 25 mM HEPES (5.9 g/L), DMEM-MAX- Without phenol Red, Low Sodium Bicarbonate (1.5 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

11 reviews for Dulbecco’s Modified Eagle’s Medium-MAX (DMEM-MAX)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

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