Eagle’s Minimal Essential Medium-MAX (EMEM-MAX)
Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. EMEM-MAX significantly improves cell viability and growth. We manufacture several types of EMEM-MAX based on your requirements.
Description
Background
Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) is a commonly used media for Mammalian cell culture.
Advantages
The advantages of EMEM-MAX over EMEM are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.
In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.
Contents
Eagle’s Minimal Essential Medium-MAX (EMEM-MAX) contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of EMEM based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
EMEM-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).
Formulation
The complete formulation can be found here: EMEM-MAX Formulation
References
- Immunization and culture of rainbow trout organ sections in vitro. Anderson DP, et al. Vet Immunol Immunopathol. 1986 Jun;12(1-4):203-11. doi: 10.1016/0165-2427(86)90124-8. PMID:
- Human plasma and amino acids as moderators of uptake and metabolic consequences of antifolates in WIL-2 and human leukemia cells. Fyfe MJ, et al. J Natl Cancer Inst. 1981 Mar;66(3):445-51. PMID:
- Dextran T-500 improves survival and spreading of chick embryo cells in serum-free medium. Pietrzkowski Z, et al. Histochem Cytobiol. 1988;26(3):123-31. PMID:
Parameter | Specification |
---|---|
Appearance | Red, clear liquid |
pH | 7.2 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | Sodium pyruvate |
Indicator | Phenol red |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Additional information
Condition | EMEM-MAX Standard Formulation, EMEM-MAX w/o Phenol Red, EMEM-MAX w/o Sodium Bicarbonate, EMEM-MAX w/o Sodium Pyruvate, EMEM-MAX w/o Glucose, EMEM-MAX w/o Phenol Red, w/o Sodium Bicarbonate, EMEM-MAX w/o Sodium Bicarbonate, w/o Sodium Pyruvate, EMEM-MAX High Sodium Bicarbonate (2.438 g/L), EMEM-MAX High Glucose (4.5 g/L), High Sodium Bicarbonate (2.438 g/L), EMEM-MAX 15 mM HEPES (3.6 g/L), EMEM-MAX 25 mM HEPES (5.9 g/L), EMEM-MAX w/o Sodium Pyruvate, w/o Phenol Red, EMEM-MAX w/o Phenol Red, with 15 mM HEPES (3.6 g/L), EMEM-MAX w/o Phenol Red, with 25 mM HEPES (5.9 g/L), EMEM-MAX w/o Phenol Red, with 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (2.438 g/L) |
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Format | Liquid, Powder |
Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
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