Earle’s Balanced Salt Solution (EBSS)

Description

Background

Earle’s Balanced Salt Solution (EBSS) is an isotonic buffer solution. It is however, contains inorganic salts and carbohydrates as an energy source designed for the short-term maintenance of mammalian cells in a CO2 environment. The solution additionally provides an ideal environment to preserve the structural and physiological integrity of cells invitro by maintaining pH as well as the balance between intra and extracellular osmotic pressure.

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

we finally sterilize the buffers by autoclaving the solution and and packding  in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics produces  the most sophisticated endotoxin removal. For that we pass the buffer  through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B   to an endotoxin-specific absorbent. we finally  eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

General Features

• It is cost effective.
• Cell Culture Suited
• This buffers maintains the cell integrity of cells or tissues during irrigation, transportation, or dilution.
• Provides an consistent environment during  the washing and re-suspend cells
• PurMa Biologics offers EBSS in three pH formats, pH 7.2, 7.4, and pH 7.6.

Specific Features

• Glucose maintains the intra and extracellular osmotic pressure as well as provides the principal source of energy for cellular metabolism.
• EBSS Provides water and important inorganic ions necessary for cellular metabolism.
• This buffer Provides a buffer solution to maintain a medium in physiological pH (7.2-7.6)
• PurMa Biologics rigorously depletes  Mg²⁺ and Ca ²⁺ from the buffers
• Free of RNAse /DNase
• Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• It is rigorously tested for contamination of endotoxin and mycoplasma.
• Is manufactured in three pH: 7.2 ± 0.01, pH: 7.4 ± 0.01, and 7.6 ± 0.01 at 25°C.
• Sterile filtered by 0.22µm membrane (in case of endotoxin-depleted EBSS by 0.1µm)
• As it is packed under N_2, the pH won’t change over time before opening the bottle.

Application

EBSS is especially has an excellent role in protocols requiring the use of consistent conditions such as crosslinking, biotinylation, and fluorescent labeling reactions which require an amine-free buffer.

Formulation

For complete formulation, click here: EBSS Formulation

References

1. Recovery from potentially lethal damage induced by spirohydantoin mustard on 9L cells in vitro. Bertrand M, Deen DF, Hoshino T, Knebel K. Cancer Treat Rep. 1980 Aug-Sep;64(8-9):889-95.
2. Rotavirus survival in conventionally treated drinking water. Sattar SA, Raphael RA, Springthorpe VS. Can J Microbiol. 1984 May;30(5):653-6. doi: 10.1139/m84-097.
3. Glucose elevates ornithine decarboxylase expression in Vero cells. Lundgren DW, Prokay SL. J Cell Physiol. 1988 Dec;137(3):469-75. doi: 10.1002/jcp.1041370311.