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Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)

$89.39$499.89

Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) is highly evaluated for endotoxin.  Moreover, Following triple filtering with 0.1µm,  the residual of endotoxin were depleted using PurMaTM Endotoxin Elimination Column (Cat# P6H1216251)

Description

Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)

Background

PurMa Biologics manufactures seven types of bovine serum, three of which however, considered as premium fetal bovine serums, each specialized for certain requirements in tissue culture. Moreover,  We highly evaluate Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) for endotoxin and after triple filtering with 0.1µm,  the residual of endo toxin were depleted using PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) ( see below).

PurMaTM Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)

  • PurMaTM Endotoxin Depleted Fetal Bovine Serum (EDP-FBS) is of USA origin.
  • Additionally, has gone through  an endotoxin depletion procedure.
  • Has gone through  gamma irradiation as well as heat inactivation process.
  • Lastly, we vigorously analyze various aspect and each LOT possess a  certificate of analysis.

Endotoxin Removal Procedure

  1. PurMa Biologics processes PurMaTM ED-FBS from PurMaTM Premium Fetal Bovine Serum (P-FBS).  It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.
  2. The process of removing endotoxin from FBS  routinely starts with passing the premium FBS through the PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) with the rate of 0.1 ml/ minute.
  3. The unbound fractions pass through 0.1µm filters  for three times. The mentioned procedure eliminates the residual of endotoxin as well.
  4. We conjugate  Sepharose 6B in our proprietary column covalently to powerful endotoxin-specific absorbent.

Analysis

PurMa biologics routinely performs pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums

References

  1. Biochemical and morphological studies of steady state and lipopolysaccaride treated bovine articular cartilage explant cultures. Tian et al. Connect Tissue Res. 1989;19(2-4):195-218. doi: 10.3109/03008208909043897. PMID:
  2. The preparation of antigenic hapten-carrier conjugates: a survey. Erlanger BF. Methods Enzymol. 1980;70(A):85-104. doi: 10.1016/s0076-6879(80)70043-5.
Methodology Unit measured Accepted level Result
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) NA Not Detected Not Detected
pH N/A measured 6.58
Osmolality (USP 785)     mOsm/KgH2 mOsm/KgH20 280-340 288
Hemoglobin (Fleming & Woolf) mg/dL <25 12.9
Endotoxin (USP 85) EU/mL Not Detected Not Detected
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) NA Not Detected Not Detected
IgG Elliza Measured 0.19 ug/ml
Albumin Eliza Measured ≤ 4.6 g/dL
Virus Testing (9 CFR 113.53c)
Bovine Adenovirus NA Not Detected Not Detected
Bovine Parvovirus NA Not Detected Not Detected
Bovine Respiratory Syncytial Virus NA Not Detected Not Detected
Bovine Viral Diarrhea Virus NA Not Detected Not Detected
Rabies NA Not Detected Not Detected
Reovirus NA Not Detected Not Detected
Cytopathogenic Agents (IBR) NA Not Detected Not Detected
Hemadsorbing Agents (PI3) NA Not Detected Not Detected
Bluetongue NA Not Detected Not Detected

 

Additional information

Size

1 x 50 ml, 1 x 500 ml

Quality Control

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Documents

Routine Pathological Analysis on PurMa Bovine and Horse Serums

SDS

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