Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)
$89.39 – $499.89
Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) is highly evaluated for endotoxin. Moreover, Following triple filtering with 0.1µm, the residual of endotoxin were depleted using PurMaTM Endotoxin Elimination Column (Cat# P6H1216251)
Description
Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)
Background
PurMa Biologics manufactures seven types of bovine serum, three of which however, considered as premium fetal bovine serums, each specialized for certain requirements in tissue culture. Moreover, We highly evaluate Endotoxin-Depleted Fetal Bovine Serum (ED-FBS) for endotoxin and after triple filtering with 0.1µm, the residual of endo toxin were depleted using PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) ( see below).
PurMaTM Endotoxin-Depleted Fetal Bovine Serum (ED-FBS)
- PurMaTM Endotoxin Depleted Fetal Bovine Serum (EDP-FBS) is of USA origin.
- Additionally, has gone through an endotoxin depletion procedure.
- Has gone through gamma irradiation as well as heat inactivation process.
- Lastly, we vigorously analyze various aspect and each LOT possess a certificate of analysis.
Endotoxin Removal Procedure
- PurMa Biologics processes PurMaTM ED-FBS from PurMaTM Premium Fetal Bovine Serum (P-FBS). It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.
- The process of removing endotoxin from FBS routinely starts with passing the premium FBS through the PurMaTM Endotoxin Elimination Column (Cat# P6H1216251) with the rate of 0.1 ml/ minute.
- The unbound fractions pass through 0.1µm filters for three times. The mentioned procedure eliminates the residual of endotoxin as well.
- We conjugate Sepharose 6B in our proprietary column covalently to powerful endotoxin-specific absorbent.
Analysis
PurMa biologics routinely performs pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums
References
- Biochemical and morphological studies of steady state and lipopolysaccaride treated bovine articular cartilage explant cultures. Tian et al. Connect Tissue Res. 1989;19(2-4):195-218. doi: 10.3109/03008208909043897. PMID:
- The preparation of antigenic hapten-carrier conjugates: a survey. Erlanger BF. Methods Enzymol. 1980;70(A):85-104. doi: 10.1016/s0076-6879(80)70043-5.
Methodology | Unit measured | Accepted level | Result |
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
pH | N/A | measured | 6.58 |
Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
Endotoxin (USP 85) | EU/mL | Not Detected | Not Detected |
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
IgG | Elliza | Measured | 0.19 ug/ml |
Albumin | Eliza | Measured | ≤ 4.6 g/dL |
Virus Testing (9 CFR 113.53c) | |||
Bovine Adenovirus | NA | Not Detected | Not Detected |
Bovine Parvovirus | NA | Not Detected | Not Detected |
Bovine Respiratory Syncytial Virus | NA | Not Detected | Not Detected |
Bovine Viral Diarrhea Virus | NA | Not Detected | Not Detected |
Rabies | NA | Not Detected | Not Detected |
Reovirus | NA | Not Detected | Not Detected |
Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
Bluetongue | NA | Not Detected | Not Detected |
Additional information
Size | 1 x 50 ml, 1 x 500 ml |
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Quality Control
[vc-quality-control-tab]SDS
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