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Endotoxin-Depleted Fetal Horse Serum (ED-FHS)

$119.89$654.59

PurMa Biologics manufactures seven types of horse serum, three of which, including PurMaTM Endotoxin-Depleted Fetal Horse Serum (EDP-FHS). Moreover, they all have the highest quality horse serums, each performs certain requirements in tissue culture.

Description

PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS)

Background

PurMa Biologics manufactures seven types of horse serum, three of which, including PurMaTM Endotoxin-Depleted Fetal Horse Serum (ED-FHS) are considered as premium fetal Horse serums, each specialized for certain requirements in tissue culture:

  1. PurMa™ Premium Fetal Horse Serum (P-FHS).
  2. PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS).
  3. PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS).

Additionally, PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS) is processed from PurMa™ Premium Fetal Horse Serum (P-FHS).  It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.

Endotoxin Removal

Purma Biologics removes the endotoxin from FHS  by passing the premium FHS through the PurMaEndotoxin Elimination Column (Cat# P6H1216251). The Sepharose 6B in our proprietary column is covalently conjugated to an endotoxin-specific absorbent. The mentioned procedure follows by eliminating the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

PurMaPremium FBS

  • Firstly, PurMa™Endotoxin Depleted Fetal Horse Serum (EDP-FHS) is of USA origin.
  • Also, it is free of endotoxin.
  • Moreover, it Has been gamma irradiated.
  • Has been heat inactivated.
  •  and lastly, has been vigorously analyzed with the certificate of analysis

Routine pathological analysis on PurMa bovine and horse serums:

Reference

  1. Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
  2. Higher percentage of horse serum in culture media blocks attachment of PC12 cells. Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.

 

Methodology Unit measured Accepted level Result
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) NA Not Detected Not Detected
pH N/A measured 6.58
Osmolality (USP 785)     mOsm/KgH2 mOsm/KgH20 280-340 288
Hemoglobin (Fleming & Woolf) mg/dL <25 12.9
Endotoxin (USP 85) EU/mL <20 <0.100
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) NA Not Detected Not Detected
IgG Elliza Measured 0.19 ug/ml
Albumin Eliza Measured ≤ 4.6 g/dL
Virus Testing
Horse Rhinopneumonitis/Equine Herpesvirus (EHV) NA Not Detected Not Detected
Horse Equine Encephalomyelitis NA Not Detected Not Detected
Horse Equine Infectious Anemia Virus (EIA) NA Not Detected Not Detected
Horse West Nile Virus NA Not Detected Not Detected
Rabies NA Not Detected Not Detected
Reovirus NA Not Detected Not Detected
Cytopathogenic Agents (IBR) NA Not Detected Not Detected
Hemadsorbing Agents (PI3) NA Not Detected Not Detected
Bluetongue NA Not Detected Not Detected

 

Additional information

Size

1 x 50 ml, 1 x 500 ml

Quality Control

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Seurm Analysis

Routine Pathological Analysis on PurMa Bovine and Horse Serums

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