Endotoxin-Depleted Fetal Horse Serum (ED-FHS)
PurMa Biologics manufactures seven types of horse serum, three of which, including PurMaTM Endotoxin-Depleted Fetal Horse Serum (EDP-FHS). Moreover, they all have the highest quality horse serums, each performs certain requirements in tissue culture.
Description
Background
PurMa Biologics manufactures seven types of horse serum, three of which, including PurMaTM Endotoxin-Depleted Fetal Horse Serum (ED-FHS) are considered as premium fetal Horse serums, each specialized for certain requirements in tissue culture:
- PurMa™ Premium Fetal Horse Serum (P-FHS).
- PurMa™ Ultra-Premium Fetal Horse Serum (UP-FHS).
- PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS).
Additionally, PurMa™ Endotoxin-Depleted Fetal Horse Serum (ED-FHS) is processed from PurMa™ Premium Fetal Horse Serum (P-FHS). It, therefore, provides an excellent opportunity to compare the two serums from an endotoxin perspective.
Endotoxin Removal
Purma Biologics removes the endotoxin from FHS by passing the premium FHS through the PurMa™ Endotoxin Elimination Column (Cat# P6H1216251). The Sepharose 6B in our proprietary column is covalently conjugated to an endotoxin-specific absorbent. The mentioned procedure follows by eliminating the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.
PurMa™ Premium FBS
- Firstly, PurMa™Endotoxin Depleted Fetal Horse Serum (EDP-FHS) is of USA origin.
- Also, it is free of endotoxin.
- Moreover, it Has been gamma irradiated.
- Has been heat inactivated.
- and lastly, has been vigorously analyzed with the certificate of analysis
Routine pathological analysis on PurMa bovine and horse serums:
Reference
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8.
- Higher percentage of horse serum in culture media blocks attachment of PC12 cells. Meth et al. 2019 Dec;67(6):256-258. Epub 2019 Oct 17.
| Methodology | Unit measured | Accepted level | Result |
| Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
| pH | N/A | measured | 6.58 |
| Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
| Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
| Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
| Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
| IgG | Elliza | Measured | 0.19 ug/ml |
| Albumin | Eliza | Measured | ≤ 4.6 g/dL |
| Virus Testing | |||
| Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
| Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
| Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
| Horse West Nile Virus | NA | Not Detected | Not Detected |
| Rabies | NA | Not Detected | Not Detected |
| Reovirus | NA | Not Detected | Not Detected |
| Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
| Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
| Bluetongue | NA | Not Detected | Not Detected |
Additional information
| Size | 1 x 50 ml, 1 x 500 ml |
|---|
Quality Control
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