Premium Fetal Bovine Serum (P-FBS)
PurMa Biologics derives Premium Fetal Bovine Serum (P-FBS) from the blood of the bovine fetus. Cell culture laboratories use FBS for growing eukaryotic especially mammalian cells. FBS contains undefined nutrition, growth factors as well. Therefore, it remains a wildly used supplement for cell culture. PurMa Biologics has developed and processes three main premium fetal bovine serums, each specialized for certain requirements in tissue culture.
Description
Background
Premium Fetal Bovine Serum (P-FBS) is derived from the blood of the bovine fetus. Cell culture laboratories use FBS for growing eukaryotic especially mammalian cells. FBS contains undefined nutrition, growth factors as well. Therefore, it remains a wildly used supplement for cell culture. PurMa Biologics has developed and processes three main premium fetal bovine serums, each specialized for certain requirements in tissue culture.
Our Serum Products
PurMa Biologics produces seven types of fetal bovine serums. The three following are considered premium:
- PurMaTM Premium Fetal Bovine Serum (P-FBS).
- PurMaTM Ultra-Premium Fetal Bovine Serum (UP-FBS).
- PurMaTM Endotoxin Depleted Fetal Bovine Serum (EDP-FBS).
PurMaTM Premium FBS
- All three PurMaTM Premium FBS are of USA origin.
- Have been gamma irradiated,
- Have been heat inactivated.
- have been vigorously analyzed with the certificate of analysis.
The major differences between P-FBS and UP-FBS
- The source of the Blood and therefore:
- Significant higher growth by UP-FBS compared to P-FBS
- The depth of the analysis:
Content of PurMaTM Premium Fetal Bovine Serum (P-FBS)
Over the years, we have tested and analyzed PurMaTM Premium Fetal Bovine Serum (P-FBS). Please see the below table. We routinely test every Lots order. We seriously look at the following criteria: IgG, Total Protein, Iron, Percent Iron Saturation, Inorganic Phosphorus, Glucose, Alkaline Phosphatase, Potassium, Albumin, Blood Urea Nitrogen, Sodium, Calcium, Chloride, Total Bilirubin, Creatinine, Gamma Globulin, , Lactate Dehydrogenase, TIBC, SGOT, SGPT.
PurMa Biologics Performs Deep Analysis
Evaluated parameters helps with the adjusting various elements , and as a result , the consistency of analysis among various Lots will highly improve.
Consistency of the ingredients among different Lots.
purifying the serum with this high stringency and robustness of the processes will make the content of serum less variable.
Analysis
Click here for the detail analysis as well as in “ Documents Tab”:Routine Pathological Analysis on PurMa Bovine and Horse Serums
References:
- The preparation of antigenic hapten-carrier conjugates: a survey. Erlanger BF. Methods Enzymol. 1980;70(A):85-104. doi: 10.1016/s0076-6879(80)70043-5. PMID:
- Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells. Hill et al. 2000 May;62(5):1135-40. doi: 10.1095/biolreprod62.5.1135. PMID: 10775159.
- Optimization of chemically defined cell culture media–replacing fetal bovine serum in mammalian in vitro methods. van der Valk et al. Toxicol In Vitro. 2010 Jun;24(4):1053-63. doi: 10.1016/j.tiv.2010.03.016. Epub 2010 Mar 31. PMID: 2036204.
Methodology | Unit measured | Accepted level | Result |
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
pH | N/A | measured | 6.58 |
Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
IgG | Elliza | Measured | 0.19 ug/ml |
Albumin | Eliza | Measured | ≤ 4.6 g/dL |
Virus Testing (9 CFR 113.53c) | |||
Bovine Adenovirus | NA | Not Detected | Not Detected |
Bovine Parvovirus | NA | Not Detected | Not Detected |
Bovine Respiratory Syncytial Virus | NA | Not Detected | Not Detected |
Bovine Viral Diarrhea Virus | NA | Not Detected | Not Detected |
Rabies | NA | Not Detected | Not Detected |
Reovirus | NA | Not Detected | Not Detected |
Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
Bluetongue | NA | Not Detected | Not Detected |
Additional information
Size | 1 x 50 ml, 1 x 500 ml |
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Quality Control
[vc-quality-control-tab]SDS
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