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Premium Fetal Bovine Serum (P-FBS)

PurMa Biologics derives Premium Fetal Bovine Serum (P-FBS)  from the blood of the bovine fetus. Cell culture laboratories use FBS for growing eukaryotic especially mammalian cells. FBS contains undefined nutrition, growth factors as well. Therefore, it remains a wildly used supplement for cell culture. PurMa Biologics has developed and processes three main premium fetal bovine serums, each specialized for certain requirements in tissue culture.

Description

Background

Premium Fetal Bovine Serum (P-FBS) is derived from the blood of the bovine fetus. Cell culture laboratories use FBS for growing eukaryotic especially mammalian cells. FBS contains undefined nutrition, growth factors as well. Therefore, it remains a wildly used supplement for cell culture. PurMa Biologics has developed and processes three main premium fetal bovine serums, each specialized for certain requirements in tissue culture.

Our Serum Products

PurMa Biologics produces seven types of fetal bovine serums. The three following are considered premium:

  1. PurMaTM Premium Fetal Bovine Serum (P-FBS).
  2. PurMaTM Ultra-Premium Fetal Bovine Serum (UP-FBS).
  3. PurMaTM Endotoxin Depleted Fetal Bovine Serum (EDP-FBS).

PurMaTM Premium FBS

  • All three PurMaTM Premium FBS are of USA origin.
  • Have been gamma irradiated,
  • Have been heat inactivated.
  • have been vigorously analyzed with the certificate of analysis.

The major differences between P-FBS and UP-FBS

  • The source of the Blood and therefore:
  • Significant higher growth by UP-FBS compared to P-FBS
  • The depth of the analysis:

Content of PurMaTM Premium Fetal Bovine Serum (P-FBS)

Over the years, we have tested and analyzed PurMaTM Premium Fetal Bovine Serum (P-FBS). Please see the below table. We routinely test  every Lots order. We seriously look at the following criteria: IgG, Total Protein, Iron, Percent Iron Saturation, Inorganic Phosphorus, Glucose, Alkaline Phosphatase,  Potassium, Albumin, Blood Urea Nitrogen, Sodium, Calcium, Chloride, Total Bilirubin, Creatinine, Gamma Globulin, , Lactate Dehydrogenase, TIBC, SGOT, SGPT.

PurMa Biologics Performs Deep Analysis

Evaluated parameters  helps with the adjusting various elements , and as a result , the consistency of analysis among various Lots will highly improve.

Consistency of the ingredients among different Lots.

purifying the serum with this high stringency and robustness of the processes will make the content of serum less variable.

Analysis

Click here for the detail analysis as well as in “ Documents Tab”:Routine Pathological Analysis on PurMa Bovine and Horse Serums

References:

  1. The preparation of antigenic hapten-carrier conjugates: a survey. Erlanger BF. Methods Enzymol. 1980;70(A):85-104. doi: 10.1016/s0076-6879(80)70043-5. PMID:
  2. Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells. Hill et al. 2000 May;62(5):1135-40. doi: 10.1095/biolreprod62.5.1135. PMID: 10775159.
  3. Optimization of chemically defined cell culture media–replacing fetal bovine serum in mammalian in vitro methods. van der Valk et al. Toxicol In Vitro. 2010 Jun;24(4):1053-63. doi: 10.1016/j.tiv.2010.03.016. Epub 2010 Mar 31. PMID: 2036204.
Methodology Unit measured Accepted level Result
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) NA Not Detected Not Detected
pH N/A measured 6.58
Osmolality (USP 785)     mOsm/KgH2 mOsm/KgH20 280-340 288
Hemoglobin (Fleming & Woolf) mg/dL <25 12.9
Endotoxin (USP 85) EU/mL <20 <0.100
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) NA Not Detected Not Detected
IgG Elliza Measured 0.19 ug/ml
Albumin Eliza Measured ≤ 4.6 g/dL
Virus Testing (9 CFR 113.53c)
Bovine Adenovirus NA Not Detected Not Detected
Bovine Parvovirus NA Not Detected Not Detected
Bovine Respiratory Syncytial Virus NA Not Detected Not Detected
Bovine Viral Diarrhea Virus NA Not Detected Not Detected
Rabies NA Not Detected Not Detected
Reovirus NA Not Detected Not Detected
Cytopathogenic Agents (IBR) NA Not Detected Not Detected
Hemadsorbing Agents (PI3) NA Not Detected Not Detected
Bluetongue NA Not Detected Not Detected

 

Additional information

Size

1 x 50 ml, 1 x 500 ml

Quality Control

[vc-quality-control-tab]

Documents

Routine Pathological Analysis on PurMa Bovine and Horse Serums

SDS

 

 

 

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