Hanks’ Balanced Salt Solution (HBSS)
Hanks’ Balanced Salt Solution (HBSS) contains inorganic salts and glucose. Moreover, HBSS for a short time provides an ideal environment to preserve the structural and physiological integrity of cells in vitro. Additionally, HBSS maintains a balance between intra and extracellular osmolarity.
Description
Background
Hanks’ Balanced Salt Solution (HBSS) contains inorganic salts and glucose. HBSS for a short time provides an ideal environment to preserve the structural and physiological integrity of cells in vitro. HBSS maintains a balance between intra and extracellular osmolarity, delivers water and inorganic ions necessary for cell metabolism, and provides energy for cellular metabolism with glucose.
Grades of Hanks’ Balanced Salt Solution (HBSS)
Regular Grade Buffer
PurMa Biologics manufactures Regular grade of buffer by using: PurMa™ Cell Culture Suited Water (Cat# P3W110101)
we finally sterilize the buffers by autoclaving the solution and packing in our Class 100 Aseptic Facility.
Cell Culture Suited Grade Buffer
PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)
In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2
RNase and DNase Free Grade Buffer
PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)
In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2
Cell Culture Suited / Endotoxin Depleted Grade Buffer
PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)
In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2
The Endotoxin Removing Procedure from Buffer
PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer through the PurMaTM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B to an endotoxin-specific absorbent. we finally eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.
General Features
- It is cost effective.
- Cell Culture Suited
- Provides maintenance and integrity for cells or tissues during irrigation, transportation, or dilution.
- To wash and re-suspend cells during the dissociation or counting process (Ca+2, Mg+2 free)
- PurMa Biologics offers HBSS in three pH formats, pH 7.2, 7.4, and pH 7.6.
Specific Features
- Glucose maintains the intra and extracellular osmotic pressure as well as provides the principal source of energy for cellular metabolism.
- HBSS Provides water and important inorganic ions necessary for cellular metabolism.
- This buffer Provides a buffer solution to maintain a medium in physiological pH (7.2-7.6)
- It is made in Mg2+ and Ca 2+-free water.
- Free of RNAse /DNase
- Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
- It is rigorously tested for contamination of endotoxin and mycoplasma.
- Is manufactured in three pH: 7.2 ± 0.01, pH: 7.4 ± 0.01, and 7.6 ± 0.01 at 25°C.
- Sterile filtered by 0.22µm membrane (in case of endotoxin-depleted buffer by 0.1µm)
- As it is packed under N2, the pH won’t change over time before opening the bottle.
Application
This buffer especially has an excellent role in protocols requiring the use of consistent conditions such as crosslinking, biotinylation, and fluorescent labeling reactions which require an amine-free buffer.
Formulation
For complete formulation, click here:HBSS Formulation
References
- Relation of oxygen and temperature in the preservation of tissues by refrigeration. Hanks, j. h., Wallace, r. e., 1949, Proc Soc Exp Biol Med. 1949 Jun;71(2):196-200. doi: 10.3181/00379727-71-17131.
- Isolation and purification of Giardia trophozoites from rat intestine. Feely DE, Erlandsen SL. J Parasitol. 1981 Feb;67(1):59-64. PMID: 7229820 .
- Selective recovery of living microfilariae from Onchocerca volvulus nodules. Determination of optimal conditions for their culture in vitro for excretory/secretory products. Ngu JL, Neba GA, Leke N, Titanji V, Asonganyi T, Ndumbe P. Acta Trop. 1981 Sep;38(3):261-6. PMID: 611803
Parameter | Specification |
---|---|
Appearance | Red, clear liquid |
pH | 6.7 ± 0.01; 7.0 ± 0.01; 7.4 ± 0.01; 7.8 ± 0.01 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | N/A |
Indicator | Phenol red |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Additional information
Concentration | 1X, 10X |
---|---|
Grade | Regular, Cell Culture Suited, Ca and Mg Free, Cell Culture Suited, Ca and Mg Free, Endotoxin Depleted |
Phenol Red | With Phenol Red, Without Phenol Red |
pH | pH 6.7, pH 7.0, pH 7.4, pH 7.8 |
Size | 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 X 1 Gallon, 6 X 1 Gallon |
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