Hanks’ Balanced Salt Solution (HBSS)

Description

Background

Hanks’ Balanced Salt Solution (HBSS) contains inorganic salts and glucose. HBSS for a short time provides an ideal environment to preserve the structural and physiological integrity of cells in vitro. HBSS maintains a balance between intra and extracellular osmolarity, delivers water and inorganic ions necessary for cell metabolism, and provides energy for cellular metabolism with glucose.

Grades of Hanks’ Balanced Salt Solution (HBSS)

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

we finally sterilize the buffers by autoclaving the solution and packing  in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer  through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B   to an endotoxin-specific absorbent. we finally  eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

General Features

• It is cost effective.
• Cell Culture Suited
• Provides  maintenance  and  integrity for  cells or tissues during irrigation, transportation, or dilution.
• To wash and re-suspend cells during the dissociation or counting process (Ca⁺², Mg⁺² free)
• PurMa Biologics offers HBSS in three pH formats, pH 7.2, 7.4, and pH 7.6.

Specific Features

• Glucose maintains the intra and extracellular osmotic pressure as well as provides the principal source of energy for cellular metabolism.
• HBSS Provides water and important inorganic ions necessary for cellular metabolism.
• This buffer Provides a buffer solution to maintain a medium in physiological pH (7.2-7.6)
• It is made in Mg²⁺ and Ca ²⁺-free water.
• Free of RNAse /DNase
• Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• It is rigorously tested for contamination of endotoxin and mycoplasma.
• Is manufactured in three pH: 7.2 ± 0.01, pH: 7.4 ± 0.01, and 7.6 ± 0.01 at 25°C.
• Sterile filtered by 0.22µm membrane (in case of endotoxin-depleted buffer by 0.1µm)
• As it is packed under N_2, the pH won’t change over time before opening the bottle.

Application

This buffer especially has an excellent role in protocols requiring the use of consistent conditions such as crosslinking, biotinylation, and fluorescent labeling reactions which require an amine-free buffer.

Formulation

For complete formulation, click here:HBSS Formulation

References

1. Relation of oxygen and temperature in the preservation of tissues by refrigeration. Hanks, j. h., Wallace, r. e., 1949, Proc Soc Exp Biol Med. 1949 Jun;71(2):196-200. doi: 10.3181/00379727-71-17131.
2. Isolation and purification of Giardia trophozoites from rat intestine. Feely DE, Erlandsen SL. J Parasitol. 1981 Feb;67(1):59-64. PMID: 7229820 .
3. Selective recovery of living microfilariae from Onchocerca volvulus nodules. Determination of optimal conditions for their culture in vitro for excretory/secretory products. Ngu JL, Neba GA, Leke N, Titanji V, Asonganyi T, Ndumbe P. Acta Trop. 1981 Sep;38(3):261-6. PMID: 611803