HEPES Stock Buffer Solution
$16.21 – $1131.62
HEPES Stock Buffer Solution adjusts the pH in cell cultures as well as biochemical analysis. HEPES Maintains psychological pH despite changes in carbon dioxide concentration, therefore HEPES it acts better than bicarbonate in cell culture.
Description
HEPES Stock Buffer Solution
Background
HEPES Stock Buffer Solution is one of the commonly used buffers in cell cultures as well as biochemical analysis. HEPES Maintains psychological pH despite changes in carbon dioxide concentration, therefore HEPES it acts better than bicarbonate in cell culture applications. However, HEPES shows an unwanted photochemical process in presence of riboflavin when exposed to ambient light to produce hydrogen peroxide. We therefore recommend keeping the mentioned solutions in dark.
Grades of HEPES Stock Buffer Solution
Regular Grade Buffer
PurMa Biologics manufactures Regular grade of buffer by using: PurMa™ Cell Culture Suited Water (Cat# P3W110101)
we finally sterilize the buffers by autoclaving the solution and packing in our Class 100 Aseptic Facility.
Cell Culture Suited Grade Buffer
PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)
In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2
RNase and DNase Free Grade Buffer
PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)
In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2
Cell Culture Suited / Endotoxin Depleted Grade Buffer
PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)
In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2
The Endotoxin Removing Procedure from Buffer
PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer through the PurMaTM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B to an endotoxin-specific absorbent. we finally eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.
Features
- Cell Culture Suited
- Provides a buffer solution to maintain the physiological pH despite changes in carbon dioxide concentration.
- PurMa Biologics manufactures this buffer using Mg2+ and Ca 2+free water
- Free of RNAse /DNase
- Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
- We rigorously test for contamination of endotoxin and mycoplasma.
- PurMa Biologics manufactures this buffer in several PH formats and NaOH adjusts the pH.
- We Sterilize this buffer using 0.2µm filters.
- As it is packed under N2, the pH won’t change over time before opening the bottle.
References
- Toxicity of light-exposed Hepes media. Lepe-Zuniga JL, Zigler JS Jr, Gery I. J Immunol Methods. 1987 Oct 23;103(1):145. doi: 10.1016/0022-1759(87)90253-5. PMID: 3655381.
- Analysis of the cytotoxic effects of light-exposed HEPES-containing culture medium. Zigler, JS Jr , Lepe-Zuniga JL, Vistica B, Gery I. In Vitro Cell Dev Biol. 1985 May;21(5):282-7. doi: 10.1007/BF02620943. PMID: 4019356.
Parameter | Specification |
---|---|
Appearance | Red, clear liquid |
pH | 6.5 ± 0.1; 7.0 ± 0.1; 7.5 ± 0.1; 7.6 ± 0.1; 8.0 ± 0.1; 8.5 ± 0.1; 9.0 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | N/A |
Indicator | N/A |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Additional information
Concentration | 0.1 M, 0.2 M, 0.5 M, 1 M, 2 M |
---|---|
Grade | Regular, RNase and DNase Free, Cell Culture Suited, Ca and Mg Free, Cell Culture Suited, Ca and Mg Free, Endotoxin Depleted |
pH | pH 6.5, pH 7.0, pH 7.5, pH 7.6, pH 8.0, pH 8.5, pH 9.0 |
Size | 1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml |
Quality Control
[vc-quality-control-tab]SDS
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