Horse Serum (HS)
Laboratories across the world use Horse Serum (HS) as a growth source for many cell types In vitro. HS provides proper growth for human Embryo Fibroblast as well as models of hepatitis. PurMa Biologics collects US origin Horse Serum (HS).
Description
Background
Laboratories around the globe use Horse Serum (HS) to support the growth of many cell types In vitro. HS provides proper growth for human Embryo Fibroblast as well as models of hepatitis. Moreover, it performs well with U937 macrophage, Human lymphocytes as well as blood cells including cord blood cells, T and B lymphocytes We collect Horse Serum (HS) from foals less than 12 months old.
Horse Serum (HS) Vs. Fetal Horse Serum (FHS)
- HS Contains approximately two folds protein compared to fetal horse serum (FHS), among them, are Iggs and other antibodies.
- Contains fewer growth factors than fetal horse serum, however, is proven to be adequate for the above-mentioned cell lines.
- Importantly, it is much more cost-effective than Fetal Horse Serum (FHS).
Special Features
- PurMa™ Horse Serum (HS) has US origin.
- PurMa™ Horse Serum (HS) has been triple-filtered through 0.1 µM filters.
- PurMa Biologics sterilizes HS with gamma irradiation methodology.
- PurMa Biologics performs heat-inactivation on our HS.
- Has been tested for mycoplasma as well as endotoxin (see below), see the certificate of analysis.
- Has been tested on multiple cell lines, such as Hek293, COS1, and NS1 cells.
Analysis
The Analysis performed on all PurMa™ Bovine and Horse Serums: Routine Pathological Analysis on PurMa™ Bovine and Horse Serums
References
- Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8. doi: 10.1007/BF02623400. PMID:
- Changes in serum influence the fatty acid composition of established cell lines. Stoll et al. In Vitro. 1984 Sep;20(9):732-8. doi: 10.1007/BF02618879. PMID:
- Serum and growth factor requirements for proliferation of human adrenocortical cells in culture: comparison with bovine adrenocortical cells. Hornsby et al. In Vitro. 1983 Nov;19(11):863-9. doi: 10.1007/BF02618166. PMID:
Methodology | Unit measured | Accepted level | Result |
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
pH | N/A | measured | 6.58 |
Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
IgG | Elliza | Measured | 0.19 ug/ml |
Albumin | Eliza | Measured | ≤ 4.6 g/dL |
Virus Testing | |||
Horse Rhinopneumonitis/Equine Herpesvirus (EHV) | NA | Not Detected | Not Detected |
Horse Equine Encephalomyelitis | NA | Not Detected | Not Detected |
Horse Equine Infectious Anemia Virus (EIA) | NA | Not Detected | Not Detected |
Horse West Nile Virus | NA | Not Detected | Not Detected |
Rabies | NA | Not Detected | Not Detected |
Reovirus | NA | Not Detected | Not Detected |
Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
Bluetongue | NA | Not Detected | Not Detected |
Additional information
Size | 1 x 50 ml, 1 x 500 ml |
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