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Horse Serum (HS)

Laboratories across the world use Horse Serum (HS) as a growth source for many cell types In vitro. HS provides proper growth for human Embryo Fibroblast as well as models of hepatitis.  PurMa Biologics collects US origin Horse Serum (HS).

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Description

Background

Laboratories around the globe use Horse Serum (HS) to support the growth of many cell types In vitro. HS provides proper growth for human Embryo Fibroblast as well as models of hepatitis. Moreover, it performs well with U937 macrophage, Human lymphocytes as well as blood cells including cord blood cells, T and B lymphocytes We collect Horse Serum (HS) from foals less than 12 months old.

Horse Serum (HS) Vs. Fetal Horse Serum (FHS)

  • HS Contains approximately two folds protein compared to fetal horse serum (FHS), among them, are Iggs and other antibodies.
  • Contains fewer growth factors than fetal horse serum, however, is proven to be adequate for the above-mentioned cell lines.
  • Importantly, it is much more cost-effective than Fetal Horse Serum (FHS).

Special Features

  • PurMa™ Horse Serum (HS) has US origin.
  • PurMa™ Horse Serum (HS) has been triple-filtered through 0.1 µM filters.
  • PurMa Biologics sterilizes HS with gamma irradiation methodology.
  • PurMa Biologics performs heat-inactivation on our HS.
  • Has been tested for mycoplasma as well as endotoxin (see below), see the certificate of analysis.
  • Has been tested on multiple cell lines, such as Hek293, COS1, and NS1 cells.

Analysis

The Analysis performed on all PurMa™ Bovine and Horse Serums: Routine Pathological Analysis on PurMa™ Bovine and Horse Serums

References

  1. Effect of horse serum on neural cell differentiation in tissue culture. Fedoroff et al. In Vitro. 1979 Aug;15(8):641-8. doi: 10.1007/BF02623400. PMID:
  2. Changes in serum influence the fatty acid composition of established cell lines. Stoll et al. In Vitro. 1984 Sep;20(9):732-8. doi: 10.1007/BF02618879. PMID:
  3. Serum and growth factor requirements for proliferation of human adrenocortical cells in culture: comparison with bovine adrenocortical cells. Hornsby et al. In Vitro. 1983 Nov;19(11):863-9. doi: 10.1007/BF02618166. PMID:
Methodology Unit measured Accepted level Result
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) NA Not Detected Not Detected
pH N/A measured 6.58
Osmolality (USP 785)     mOsm/KgH2 mOsm/KgH20 280-340 288
Hemoglobin (Fleming & Woolf) mg/dL <25 12.9
Endotoxin (USP 85) EU/mL <20 <0.100
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) NA Not Detected Not Detected
IgG Elliza Measured 0.19 ug/ml
Albumin Eliza Measured ≤ 4.6 g/dL
Virus Testing
Horse Rhinopneumonitis/Equine Herpesvirus (EHV) NA Not Detected Not Detected
Horse Equine Encephalomyelitis NA Not Detected Not Detected
Horse Equine Infectious Anemia Virus (EIA) NA Not Detected Not Detected
Horse West Nile Virus NA Not Detected Not Detected
Rabies NA Not Detected Not Detected
Reovirus NA Not Detected Not Detected
Cytopathogenic Agents (IBR) NA Not Detected Not Detected
Hemadsorbing Agents (PI3) NA Not Detected Not Detected
Bluetongue NA Not Detected Not Detected

 

Additional information

Size

1 x 50 ml, 1 x 500 ml

Quality Control

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Documents

Routine Pathological Analysis on PurMa Bovine and Horse Serums

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