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Leibovitz’s L-15 Medium – MAX

Leibovitz’s L-15 Medium – MAX significantly improves the viability and growth of cells.  It additionally contains L-Alanyl-L-Glutamine and therefore supplies a longer shelf life.  Moreover, we manufacture several types of Leibovitz’s L-15 MAX based on your requirements.

Description

Background

Leibovitz’s L-15 Medium – MAX is one of the commonly used Media for Mammalian cell culture.

Advantages

The advantages of Leibovitz’s L-15 MAX over Leibovitz’s L-15 are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. Therefore, one way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture.  Unfortunately, maintaining a consistent level of L-glutamine by tracking time can be a tedious and potentially impossible task.

Alternatively, L-alanyl-L-glutamine can be used since it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Contents

Leibovitz’s L-15 Medium – MAX contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of Leibovitz’s L-15 MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

Leibovitz’s L-15 MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation is here: Leibovitz’s L-15 MAX Formulation

References

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

Leibovitz’s L-15-MAX Standard Formulation, Leibovitz’s L-15-MAX w/o Phenol Red, Leibovitz’s L-15-MAX w/o Sodium Pyruvate, Leibovitz’s L-15-MAX w/o Galactose, Leibovitz’s L-15-MAX High Galactose (4.5 g/L), Leibovitz’s L-15-MAX 15 mM HEPES (3.6 g/L), Leibovitz’s L-15-MAX 25 mM HEPES (5.9 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

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