Lipid-Depleted Fetal Bovine Serum (LD-FBS)
$166.36 – $1339.99
Generally, the best used for Lipid-Depleted Fetal Bovine Serum (LD-FBS) in research applications is when the normal concentration of lipids may interfere with the results. moreover, For studies directly involve lipid metabolism. That is because the composition of serum can have a profound impact on important cellular checkpoints. It is also used for the generation of media with defined lipid species.
Description
Lipid-Depleted Fetal Bovine Serum (LD-FBS)
Background
The analysis of lipid metabolism in cultured cells is challenging. That is because the cell culture carries out in presence of animal serum, which contains a wide range of undefined lipid species. Generally, the best used for Lipid-Depleted Fetal Bovine Serum (LD-FBS) in research applications is:
- When the normal concentration of lipids may interfere with the results.
- For studies directly involve lipid metabolism. That is because the composition of serum can have a profound impact on important cellular checkpoints.
- It is used for the generation of media with defined lipid species.
Special Features
- PurMaTM Premium LD-FBS is of USA origin
- Additionally, has gone through an endotoxin depletion procedure.
- Has gone through gamma irradiation as well as heat inactivation process.
- PurMa Biologics performs the most sophisticated lipid depletion.
- Th process will continue with dialysis using the 3500 Dalton dialysis tube.
- The dialysis carries out against 5X 10 Liters of PBD with physiological pH per each 500 ml of serum.
- The procedure depletes the lipids, phospholipids, and a long chain of fatty acids.
- Lastly, we vigorously analyze various aspects, and each LOT possesses a certificate of analysis.
- The process eliminate mycoplasma, IgG, steroids and hormones, and other pathogens.
- Acceptable level of Endotoxin hemoglobin (20 mg/DL) and endotoxin (10 mg/DL).
Analysis
An example of results following PurMa™ FBS Lipid depletion procedure : The Lipid Analysis of Lot: 30143050032423 following Lipid Depletion
For non-proprietary information in PurMa biologics’ general procedure for FBS Lipid depletion, click here: PurMa™ FBS Lipid depletion Procedure
Routine pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums
References
- Effect of the removal of lipids on specific precipitation. Krueger et al. J Exp Med. 1950 Oct 31; 92(5): 383–391. doi: 10.1084/jem.92.5.383. PMCID: PMC2135985.
- Preparation of Lipid-Stripped Serum for the Study of Lipid Metabolism in Cell Culture. Aaron et al. 2018 Jun 5; 8(11): e2876. Published online 2018 Jun 5.doi:10.21769/Bio Pro toc.2876, PMCID: PMC8275250.
Methodology | Unit measured | Accepted level | Result |
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
pH | N/A | measured | 6.58 |
Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
IgG | Elliza | Measured | 0.19 ug/ml |
Albumin | Eliza | Measured | ≤ 4.6 g/dL |
Virus Testing (9 CFR 113.53c) | |||
Bovine Adenovirus | NA | Not Detected | Not Detected |
Bovine Parvovirus | NA | Not Detected | Not Detected |
Bovine Respiratory Syncytial Virus | NA | Not Detected | Not Detected |
Bovine Viral Diarrhea Virus | NA | Not Detected | Not Detected |
Rabies | NA | Not Detected | Not Detected |
Reovirus | NA | Not Detected | Not Detected |
Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
Bluetongue | NA | Not Detected | Not Detected |
Additional information
Size | 1 x 50 ml, 1 x 500 ml |
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Quality Control
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