Lipid-Depleted Fetal Bovine Serum (LD-FBS)
Generally, the best used for Lipid-Depleted Fetal Bovine Serum (LD-FBS) in research applications is when the normal concentration of lipids may interfere with the results. moreover, For studies directly involve lipid metabolism. That is because the composition of serum can have a profound impact on important cellular checkpoints. It is also used for the generation of media with defined lipid species.
Description
Background
The analysis of lipid metabolism in cultured cells is challenging. That is because the cell culture carries out in presence of animal serum, which contains a wide range of undefined lipid species. Generally, the best used for Lipid-Depleted Fetal Bovine Serum (LD-FBS) in research applications is:
- When the normal concentration of lipids may interfere with the results.
- For studies directly involve lipid metabolism. That is because the composition of serum can have a profound impact on important cellular checkpoints.
- It is used for the generation of media with defined lipid species.
Special Features
- PurMaTM Premium LD-FBS is of USA origin
- Additionally, has gone through an endotoxin depletion procedure.
- Has gone through gamma irradiation as well as heat inactivation process.
- PurMa Biologics performs the most sophisticated lipid depletion.
- Th process will continue with dialysis using the 3500 Dalton dialysis tube.
- The dialysis carries out against 5X 10 Liters of PBD with physiological pH per each 500 ml of serum.
- The procedure depletes the lipids, phospholipids, and a long chain of fatty acids.
- Lastly, we vigorously analyze various aspects, and each LOT possesses a certificate of analysis.
- The process eliminate mycoplasma, IgG, steroids and hormones, and other pathogens.
- Acceptable level of Endotoxin hemoglobin (20 mg/DL) and endotoxin (10 mg/DL).
Analysis
An example of results following PurMa™ FBS Lipid depletion procedure : The Lipid Analysis of Lot: 30143050032423 following Lipid Depletion
For non-proprietary information in PurMa biologics’ general procedure for FBS Lipid depletion, click here: PurMa™ FBS Lipid depletion Procedure
Routine pathological analysis on PurMa bovine and horse serums: Routine Patalogicsl Analysis on PurMa Bovine and Horse Serums
References
- Effect of the removal of lipids on specific precipitation. Krueger et al. J Exp Med. 1950 Oct 31; 92(5): 383–391. doi: 10.1084/jem.92.5.383. PMCID: PMC2135985.
- Preparation of Lipid-Stripped Serum for the Study of Lipid Metabolism in Cell Culture. Aaron et al. 2018 Jun 5; 8(11): e2876. Published online 2018 Jun 5.doi:10.21769/Bio Pro toc.2876, PMCID: PMC8275250.
Methodology | Unit measured | Accepted level | Result |
Sterility (Current USP and EP 2.6.1 for Bacteria & Fungi) | NA | Not Detected | Not Detected |
pH | N/A | measured | 6.58 |
Osmolality (USP 785) mOsm/KgH2 | mOsm/KgH20 | 280-340 | 288 |
Hemoglobin (Fleming & Woolf) | mg/dL | <25 | 12.9 |
Endotoxin (USP 85) | EU/mL | <20 | <0.100 |
Mycoplasma (Barile & Kern; Large Volume, Direct Culture) | NA | Not Detected | Not Detected |
IgG | Elliza | Measured | 0.19 ug/ml |
Albumin | Eliza | Measured | ≤ 4.6 g/dL |
Virus Testing (9 CFR 113.53c) | |||
Bovine Adenovirus | NA | Not Detected | Not Detected |
Bovine Parvovirus | NA | Not Detected | Not Detected |
Bovine Respiratory Syncytial Virus | NA | Not Detected | Not Detected |
Bovine Viral Diarrhea Virus | NA | Not Detected | Not Detected |
Rabies | NA | Not Detected | Not Detected |
Reovirus | NA | Not Detected | Not Detected |
Cytopathogenic Agents (IBR) | NA | Not Detected | Not Detected |
Hemadsorbing Agents (PI3) | NA | Not Detected | Not Detected |
Bluetongue | NA | Not Detected | Not Detected |
Additional information
Size | 1 x 50 ml, 1 x 500 ml |
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Quality Control
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