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Mammalian Cryoprotective Media (MCM-D) with DMSO

Mammalian Cryoprotective Media (MCM-D) with DMSO

(11 customer reviews)

Mammalian Cryoprotective Media (MCM-D) with DMSO provides the best cryoprotection. Moreover, it provides an equilibrium that results in the lowest cell death with an excellent recovery rate (69%).

SKU: N/A Categories: Cryoprotective media With DMSO, Cryoprotective Media Tags: With DMSO, Mammalian Cells, Cryprotective media

Description

Background

Mammalian Cryoprotective Media (MCM-D) with DMSO provides the best cryoprotection with proven 69% recovery rate (see below). Exposing cells to temperatures below 0°C without the aid of cryoprotectants typically cause cell death. During cryopreservation cooling aqueous solutions below the equilibrium freezing point inevitably result in the formation of ice crystals in the extracellular media and a decrease in the concentration of water which has two key consequences:

  1. Osmotic gradient forms across the cell membrane, which causes “cell dehydration”, and
  2. Ice formation and abnormal concentration of solutes in the intracellular matrix, osmatic shock, and toxicity.

More Importantly, we always manufacture  our cryoprotective media using our most sophisticated water , for more details, Click here:

PurMa™ Ultra-Pure Cell Culture Suited Water

PurMa Biologics is the leading company in manufacturing cryoprotective media. We follow two essential goals:

  1. Allowing cells to be stored at deep cryogenic temperatures with minimal cell damage and recovery with high levels of appropriate functionality and viability, and
  2. Allow minimal toxicity.  Undoubtedly, this is a challenge we have overcome in the past 3 decades.

Accordingly, we manufacture several DMSO -dependent and DMSO Free cryoprotective media. PurMaTM (MCM) contains DMSO.

PurMaTM Mammalian Cryoprotective Media (MCM) has an excellent performance for short-term cold storage as well as long-term cryopreservation. Please see the below figure

DMSO benefits cryoprotection and cryoprotective agent in several ways

  1. Provides strong molecular stability.
  2. Besides, facilitates a high non-conductor constant.
  3. It contains the basic properties, salts solubility particularly for anions. Additionally contains a propensity to act as the acceptor atom in hydrogen bonding.
  4. Especially,  DMSO makes a  hydrogen bond with water which is 30% stronger than between two water molecules.
  5. The proprietary formulation of PurMaTM Mammalian Cryoprotective Media (MCM) allows the MCM to get inside the cells instantly and lower cell death because of penetration/freezing.

Special Features

  • Ready-to-Use medium.
  • Each Lot of our Mammalian Cryoprotective Media (MCM-D) with DMSO goes through pathogens and sterility evaluations.
  • We evaluate each Lot for survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), as well as for the rate of recovery by Alamar Blue test.
  • PurMaTM MCM-D results in the lowest cell loss during freezing and thawing as well as the highest cell recovery. Moreover, it provides over 69% survival and recovery rate after long term cryopreservation.
  • Sterile (Passed through 0.2µm filters).
  • Moreover, PurMaTM MCM-D provides an exceptional  cryopreservation for primary and commercial cell lines
  • Suitable for clinical applications.

Guideline

For PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines

References

  1. The chemical and physical properties of DMSO. MacGregor WS. Ann N Y Acad Sci. 1967 Mar 15;141(1):3-12. doi: 10.1111/j.1749-6632.1967.tb34860.x. PMID: 5342259.
  2. Polyampholytes as cryoprotective agents for mammalian cell cryopreservation. Matsumura et al. Cell Transplant. 2010;19(6):691-9. doi: 10.3727/096368910X508780. Epub 2010 Jun 3. PMID: 20525437.
Parameter Specification
Appearance Red liquid
pH 7.4 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Sodium pyruvate NA
Indicator NA
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition Dry Ice

 

Additional information

Size

1 x 10 ml, 1 x 100 ml

11 reviews for Mammalian Cryoprotective Media (MCM-D) with DMSO

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

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