McCoy’s 5A Modified Media MAX (M5AMM-MAX)
$31.51 – $573.22
McCoy’s 5A Modified Media MAX (M5AMM-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. McCoy’s 5A MAX significantly improves cell viability and growth. We manufacture several types of McCoy’s 5A MAX based on your requirements.
Description
McCoy’s 5A Modified Media-MAX (M5AMM-MAX)
Background
McCoy’s 5A Modified Media MAX (M5AMM-MAX) is a commonly used media for Mammalian cell culture.
Advantages
The advantages of McCoy’s 5A MAX over McCoy’s 5A are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.
In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.
Contents
McCoy’s 5A Modified Media MAX (M5AMM-MAX) contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of McCoy’s 5A based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
McCoy’s 5A MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).
Formulation
The complete formulation can be found here: McCoy’s 5A MAX Formulation
References
- Mycoplasmal infection of insect cell cultures. Steiner T, McGarrity G. In Vitro. 1983 Sep;19(9):672-82. doi: 10.1007/BF02628958.PMID: 6413389
- Investigations on in vitro survival and virulence of T. pallidum under aerobiosis. Rathlev T. Br J Vener Dis. 1975 Oct;51(5):296-300. doi: 10.1136/sti.51.5.296. PMID: 172190
- Altered polyamine metabolism in Chinese hamster cells growing in a defined medium. Sertich GJ, et al. J Cell Physiol. 1986 Apr;127(1):114-20. doi: 10.1002/jcp.1041270115.PMID: 3958058
Parameter | Specification |
---|---|
Appearance | Red, clear liquid |
pH | 7.2 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | Sodium pyruvate |
Indicator | Phenol red |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Additional information
Condition | M5AMM-MAX – Standard Formulation, M5AMM-MAX -Without Phenol Red, M5AMM-MAX -Without Sodium Bicarbonate, M5AMM-MAX – Without Glucose, M5AMM-MAX – Without Peptone, M5AMM-MAX – Without Phenol Red, Without Sodium Bicarbonate, M5AMM-MAX – Low Glucose (1.5 g/L), M5AMM-MAX – Low Sodium Bicarbonate (0.35 g/L), M5AMM-MAX – Low Sodium Bicarbonate (0.85 g/L), M5AMM-MAX -15 mM HEPES (3.6 g/L), M5AMM-MAX – 25 mM HEPES (5.9 g/L) |
---|---|
Format | Liquid, Powder |
Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
SDS
SDS
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