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MEM Alpha-Modification MAX in Earle’s Buffer (MEM-Alpha-MAX)

MEM Alpha-Modification MAX (MEM-α-MAX) in Earle’s Buffer contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life.   MEM-α-MAX in EBBS significantly improves cell viability and growth. We manufacture several types of MEM-α-MAX based on your requirements.

Description

Background

MEM Alpha-Modification MAX (MEM-α-MAX) in Earle’s Buffer is a commonly used media for Mammalian cell culture.

Advantages

MEM-α performs better than MEM for a number of cell lines, please see the description of regular MEM-α: MEM-a in Earle’s Buffer

The advantages of MEM-α-MAX in EBBS over MEM-α are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, generating toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Contents

MEM Alpha-Modification MAX (MEM-α-MAX) in Earle’s Buffer contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of MEM-α-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

MEM-α-MAX in EBBS contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

The complete formulation can be found here: MEM-alpha-MAX Formulation in Earl’s Buffer

References

  1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
  2. Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, Wun CC, Grunert GM. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
  3. Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham’s F-10. Roudebush WE, Often NL, Butler WJ. J Assist Reprod Genet. 1994 Apr;11(4):203-7. doi: 10.1007/BF02211809. PMID: 7711383.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM-α-MXA Standard Formulation, MEM-α-MXA w/o Phenol Red, MEM-α-MXA w/o Sodium Bicarbonate, MEM-α-MXA w/o Glucose, MEM-α-MXA w/o Lipoic Acid, MEM-α-MXA 15 mM HEPES (3.6 g/L), MEM-α-MXA 25 mM HEPES (5.9 g/L), MEM-α-MXA w/o Sodium Pyruvate, MEM-α-MXA High Glucose (4.5 g/L), MEM-α-MXA High Sodium Bicarbonate (3.7 g/L), MEM-α-MXA Low Sodium Bicarbonate (0.85 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

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