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MEM Alpha Modification (MEM-α) in Hanks’ Buffer

MEM Alpha Modification (MEM-α) in Hanks’ Buffer  is suitable  for mammalian cell culture . Moreover, This media can also be used with a variety of suspension as well as  adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.

Description

Background

MEM Alpha Modification (MEM-α) in Hanks’ Buffer is certainly a suitable choice for mammalian cell culture as well as selection for transfected DHFR-negative cells. This media can also be used with a variety of suspension and adherent cells including keratinocytes, primary rat astrocytes, and human melanoma cells.  furthermore it is important that eliminating calcium in this media facilitates the growth of cells in suspension cultures.

Ingredients

MEM -α has improved regular MEM as it contains:

  • Non-essential amino acids.
  • Sodium pyruvate.
  • Lipoic acid.
  • Vitamin B12.
  • Biotin and,
  • Ascorbic acid.
  • Also, MEM-α does not contain deoxyribonucleosides and ribonucleosides.

PurMa™ MEM -α media in Hank’s buffer includes HBSS

Composition of HBSS buffer contains:

  • Potassium Chloride
  • Potassium Phosphate, monobasic
  • Sodium Bicarbonate
  • Sodium Chloride
  • Sodium Phosphate, dibasic
  • Low level of glucose (1.00 g/ liter)
  • Sodium bicarbonate (0.35 g/L)
  • 2 mM L-glutamine (292 mg/L)
  • Low level of glucose (1.00 g/ liter)

Furthermore, PurMa Biologics manufactures 123 types of MEM with HBSS buffer

Why PurMa Biologics? Why we stand behind our cell culture media

Our products are the outcome of 30 years of experience in cutting edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonabl

Application

Alpha modification of Minimum Essential Medium Eagle certainly makes an excellent for the isolation of mesenchymal stem cells, adipose-derived stem cells as well as osteoblast. MEM -α Modification is widely used for primary osteoblast cultures as well as isolation of polymorphonuclear leukocytes (PMN) from umbilical cord blood.

Formulation

to get access to detailed formulation visit ” formulation Tab” as well as by clicking here : MEM-alpha Formulation in Hank’s Buffer (HBSS)

References

  1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay L.  Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
  2. Sphingosine-1-phosphate lyase (SGPL1) deficiency is associated with mitochondrial dysfunction. Maharaj et al. The Journal of steroid biochemistry and molecular biology, 202, 105730-105730 (2020-07-20)

 

Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM-α (HBSS) Standard Formulation, MEM-α (HBSS) w/o Glutamine, MEM-α (HBSS) w/o Phenol Red, MEM-α (HBSS) w/o Sodium Bicarbonate, MEM-α (HBSS) w/o Glucose, MEM-α (HBSS) w/o Lipoic Acid, MEM-α (HBSS) w/o Glutamine, w/o Phenol Red, MEM-α (HBSS) w/o Glutamine, w/o Sodium Bicarbonate, MEM-α (HBSS) w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α (HBSS) 15 mM HEPES (3.6 g/L), MEM-α (HBSS) 25 mM HEPES (5.9 g/L), MEM-α (HBSS) w/o Sodium Pyruvate, MEM-α (HBSS) High Glucose (4.5 g/L), MEM-α (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-α (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Quality Control

[vc-quality-control-tab]

Formulation

MEM-α in HBSS Buffer Formulation

 

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