MEM-Alpha-Modification with Nucleosides in Hanks’ Buffer Test Seif
$29.93 – $715.78
MEM α-Modification with Nucleosides in Hanks’ buffer provides a great nutritional culture environment for mammalian cells. this media as well plays a key role in the process of selection for transfected DHFR-negative cells.
Description
MEM α-Modification with Nucleosides in Hanks’ Buffer
Background
MEM α-Modification with Nucleosides in Hanks’ buffer provides a great nutritional culture environment during mammalian cells. This media, as well plays a key role in the process of selection of transfected DHFR-negative cells. Moreover, MEM-α-N-HBSS is widely used for a variety of suspension and adherent cells. Calcium, however, plays a crucial role in media as eliminating this ion facilitates the growth of cells in suspension cultures.
Ingredients
MEM-α with nucleosides is basically the modified version of MEM-α and contains:
- Ribonucleosides
- Deoxyribonucleosides
- Non-essential amino acids.
- Sodium pyruvate.
- Lipoic acid.
- Vitamin B12.
- Biotin and,
- Ascorbic acid.
PurMa™ MEM-α-N-HBSS contains HBSS buffer
The composition of HBSS buffer:
- Potassium Chloride
- Potassium Phosphate, monobasic
- Sodium Bicarbonate
- Sodium Chloride
- Sodium Phosphate, dibasic
Additionally, MEM-α-N-HBSS also Contains:
- Low level of glucose (1.00 g/ liter)
- Sodium bicarbonate (0.35 g/L)
- 2 mM L-glutamine (292 mg/L)
PurMa Biologics manufactures 119 types of MEM-α-N-HBSS
Why PurMa Biologics? Why we stand behind our cell culture media
Our products are the outcome of 30 years of experience in cutting-edge science. We routinely manufacture over 1500 media. We have the most comprehensive collection of cell and tissue culture media. Moreover, We utilize %99 pure amino acids. This will significantly increase the consistency among your experiments. Considering the highest quality, our prices certainly are extremely reasonable.
Application
Furthermore, MEM-α-N-HBSS is used for various cell types such as keratinocytes, primary rat astrocytes, as well as human melanoma cells. also, MEM-α-N-HBSS has been used for the isolation of mesenchymal stem cells, adipose-derived stem cells, and osteoblasts. Moreover, MEM α-Modification is widely used for primary osteoblast cultures.
Formulation
For complete formulation click here as well as ” formulation Tab : MEM alpha with nucleoside in HBSS Buffer Formulation
References
- A previously functional tetracycline-regulated transactivator fails to target gene expression to the bone. Schmidt et al. BMC Res Notes. 2011 Aug 11;4:282. doi: 10.1186/1756-0500-4-282. PMID: 21835026.
- The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. Keay L, Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | MEM-α-N (HBSS) Standard Formulation, MEM-α-N (HBSS) w/o Glutamine, MEM-α-N (HBSS) w/o Phenol Red, MEM-α-N (HBSS) w/o Sodium Bicarbonate, MEM-α-N (HBSS) w/o Glucose, MEM-α-N (HBSS) w/o Lipoic Acid, MEM-α-N (HBSS) w/o Glutamine, w/o Phenol Red, MEM-α-N (HBSS) w/o Glutamine, w/o Phenol Red, w/o Sodium Bicarbonate, MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), MEM-α-N (HBSS) w/o Sodium Pyruvate, MEM-α-N (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-α-N (HBSS) High Glucose (4.5 g/L), MEM-α-N (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α-N (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L) |
|---|---|
| Format | Liquid, Powder |
| Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
SDS
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