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MEM Alpha-Modification with Nucleosides MAX in Earle’s Buffer (MEM-Alpha-N-MAX)

$41.61$663.94

MEM α-Modification with Nucleosides MAX in Earle’s Buffer (MEM-α-N-MAX-EBBS) contains L-Alanyl-L-Glutamine.   Additionally, MEM-α-N-MAX (EBBS) significantly improves cell viability and growth. We manufacture several types of MEM-α-N-MAX based on your requirements.

Description

MEM α-Modification with Nucleosides MAX in Earle’s Buffer (MEM-α-N-MAX-EBBS)

Background

MEM α-Modification with Nucleosides MAX in Earle’s Buffer (MEM-α-N-MAX-EBBS) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of MEM-α-N-MAX over MEM-α-N are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Consequently, it improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine that prevents the degradation of L-glutamine.

Contents

MEM α-Modification with Nucleosides MAX in Earle’s Buffer (MEM-α-N-MAX-EBBS) contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of MEM-α-N-MAX (EBBS) based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

PurMa™ MEM-α with nucleosides MAX (MEM-α-N-MAX) is modified from MEM-α-MAX with the following ingredients:

  • Ribonucleosides
  • Deoxyribonucleosides

MEM-α-N-MAX (EBBS) contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

For complete formulation Click here: MEM alpha-MAX with Nucleoside Formulation

References

  1. The growth of L-cells and Vero cells on an autoclavable MEM-peptone medium. L Keay Biotechnology and bioengineering, 19(3), 399-411 (1977-03-01).
  2. Minimum essential medium alpha (MEM) enhances assisted reproductive technology results. I. Mouse embryo study. Wun WS, et al. J Assist Reprod Genet. 1994 Jul;11(6):303-7. doi: 10.1007/BF02215717. PMID: 7734915.
  3. Alpha-minimum essential medium (MEM) enhances in vitro hatched blastocyst development and cell number per embryo over Ham’s F-10. Roudebush WE, Often NL, Butler WJ. J Assist Reprod Genet. 1994 Apr;11(4):203-7. doi: 10.1007/BF02211809. PMID: 7711383.
Parameter Specification
Appearance Red, clear liquid
pH 7.2 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability  Suitable for mammalian cell culture
Additive Sodium pyruvate
Indicator Phenol red
Mycoplasma Detection Negative
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition  Room temperature

 

Additional information

Condition

MEM-α-N -MAX Standard Formulation, MEM-α-N -MAX w/o Phenol Red, MEM-α-N -MAX w/o Sodium Bicarbonate, MEM-α-N -MAX w/o Glucose, MEM-α-N -MAX w/o Lipoic Acid, MEM-α-N -MAX 15 mM HEPES (3.6 g/L), MEM-α-N -MAX 25 mM HEPES (5.9 g/L), MEM-α-N -MAX w/o Sodium Pyruvate, MEM-α-N -MAX High Glucose (4.5 g/L), MEM-α-N -MAX Low Sodium Bicarbonate (0.35 g/L), MEM-α-N -MAX Low Sodium Bicarbonate (0.85 g/L)

Format

Liquid, Powder

Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

Quality Control

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Formulation

MEM-α-N-MAX in Earl’s Buffer

 

SDS

SDS

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