MEM Alpha–Modification with Nucleosides MAX (MEM-Alpha–N-MAX) in Hanks’ Buffer
$30.74 – $647.87
MEM α-Modification with Nucleosides MAX in Hanks’ Buffer (MEM-α-N-MAX-HBSS) contains L-Alanyl-L-Glutamine, as well as Nucleosides. Additionally, MEM-α-N-MAX (HBSS) significantly improves cell viability and growth. We manufacture several types of MEM-α-N-MAX based on your requirements.
Description
MEM α-Modification with Nucleosides MAX in Hanks’ Buffer
(MEM-α-N-MAX-HBSS)
Background
MEM α-Modification with Nucleosides MAX in Hanks’ Buffer (MEM-α-N-MAX-HBSS) is a commonly used media for Mammalian cell culture.
Advantages
The advantages of MEM-α-N-MAX (HBSS) over MEM-α-N (HBSS) are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Consequently, it improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.
In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.
Contents
MEM α-Modification with Nucleosides MAX in Hanks’ Buffer (MEM-α-N-MAX-HBSS) contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of MEM-α-N-MAX (HBSS) based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
PurMa™ MEM-α with nucleosides MAX (MEM-α-N-MAX) is modified from MEM-α-MAX with the following ingredients:
- Ribonucleosides
- Deoxyribonucleosides
MEM-α-N-MAX (HBSS) contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).
Formulation
The complete formulation can be found here: MEM alpha-MAX with nucleoside in HBSS Buffer Formulation
References
- Culture of granulosa cells in collagen gels: the influence of cell shape on steroidogenesis. Carnegie JA, et al. Biol Reprod. 1988 May;38(4):881-90. doi: 10.1095/biolreprod38.4.881. PMID: 3401543
- Myelin formation in rat dorsal root ganglion cultured in a serum-free medium. Ninomiya T, Takahashi K. J Anat. 1987 Jun;152:209-13. PMID: 3654371
- Long-term organ culture of hamster cheek pouch mucosa. Mock D, Main JH. J Oral Pathol. 1976 Jul;5(4):237-40. doi: 10.1111/j.1600-0714.1976.tb01770.x. PMID: 820846
Parameter | Specification |
---|---|
Appearance | Red, clear liquid |
pH | 7.2 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | Sodium pyruvate |
Indicator | Phenol red |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Additional information
Condition | MEM-α-N-MAX (HBSS) Standard Formulation, MEM-α-N-MAX (HBSS) w/o Phenol Red, MEM-α-N-MAX (HBSS) w/o Sodium Bicarbonate, MEM-α-N-MAX (HBSS) w/o Glucose, MEM-α-N-MAX (HBSS) w/o Lipoic Acid, MEM-α-N-MAX (HBSS) 15 mM HEPES (3.6 g/L), MEM-α-N-MAX (HBSS) 25 mM HEPES (5.9 g/L), MEM-α-N-MAX (HBSS) w/o Sodium Pyruvate, MEM-α-N-MAX (HBSS) High Sodium Bicarbonate (0.85 g/L), MEM-α-N-MAX (HBSS) High Glucose (4.5 g/L), MEM-α-N-MAX (HBSS) 15 mM HEPES (3.6 g/L), High Glucose (4.5 g/L), MEM-α-N-MAX (HBSS) 25 mM HEPES (5.9 g/L), High Glucose (4.5 g/L) |
---|---|
Format | Liquid, Powder |
Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
SDS
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