PurMa EDTA Buffer Solution
$13.60 – $472.54
PurMa Biologics manufactures various grades of EDTA Buffer solution using different grades of water. Moreover, EDTA solution is suitable for biochemistry or molecular biology applications requiring a chelator of divalent metal ions. In fact, we adjust the pH to 8.0 by sodium hydroxide.
Description
EDTA Buffer
Background
PurMa Biologics manufactures various grades of PurMa EDTA Buffer Solution NaEDTA•2HO using different grades of water ( see below). Moreover, EDTA solution is suitable for biochemistry or molecular biology applications requiring a chelator of divalent metal ions. Additionally, we adjust the pH to 8.0 with sodium hydroxide.
Grades of EDTA Buffer
Regular Grade Buffer
PurMa Biologics manufactures Regular grade of buffer by using: PurMa™ Cell Culture Suited Water (Cat# P3W110101)
we finally sterilize the buffers by autoclaving the solution and packing in our Class 100 Aseptic Facility.
Cell Culture Suited Grade Buffer
PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)
In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2
RNase and DNase Free Grade Buffer
PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)
In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2
Cell Culture Suited / Endotoxin Depleted Grade Buffer
PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)
In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2
The Endotoxin Removing Procedure from Buffer
PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer through the PurMaTM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B to an endotoxin-specific absorbent. we finally eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.
Features
- Cell Culture Suited.
- provides a cost-effective and easy to use buffer.
- Suitable for a variety of biochemical applications.
- EDTA chelates heavy metals such as Mg2+ and Ca 2+.
- PurMa Biologics manufactures EDTA in Mg2+ and Ca 2+-free water.
- Free of RNAse /DNase
- Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
- It is rigorously tested for contamination of endotoxin and mycoplasma.
- Is manufactured in two pH formats: pH: 8.00 ± 0.01and 8.5 ± 0.01 at 25°C.
- Sterile filtered by 0.22µm membrane (in case of endotoxin depleted HBSS by 0.1µm)
- It is packed under N2; therefore, the pH won’t change over time before opening the bottle.
Formulation
For complete formulation, click here:EDTA Formulation
References
- Purification of two endonucleases from potato tubers. BJOERK W. Biochim Biophys Acta. 1965 Apr 19;95:652-62. PMID: 14324816.
- Chelating agents. Bergan T, Klaveness J, Aasen AJ. Chemotherapy. 2001 Jan-Feb;47(1):10-4. doi: 10.1159/000048495. PMID: 11125227.
Parameter | Specification |
---|---|
Appearance | Clear liquid |
pH | 8.0 ± 0.1; 8.5 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | N/A |
Indicator | N/A |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Additional information
Concentration | 0.1 M, 0.5 M, 1 M |
---|---|
Grade | Regular, RNase and DNase Free, Cell Culture Suited, Ca and Mg Free, Cell Culture Suited, Ca and Mg Free, Endotoxin Depleted |
pH | pH 8.0, pH 8.5 |
Size | 1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml |
Quality Control
[vc-quality-control-tab]Formulation
SDS
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