Description

PurMa TRIS Buffer Solution

Background

PurMa TRIS Buffer Solution is frequently used buffers in molecular biology, cell culture and protein biochemistry. In fact, it provides a buffering capacity  in solutions. Moreover, PurMa TRIS Buffer Solution has an important impact for nucleic acid applications. It contains a primary amine and thus undergoes the reactions associated with typical amines, e.g., condensations with aldehydes. The pH of  tris buffer has an important impact on its function .

Grades of Tris Buffer Solutions

Regular Grade Buffer

PurMa Biologics manufactures Regular grade of buffer by using:  PurMa™ Cell Culture Suited Water (Cat# P3W110101)

we finally sterilize the buffers by autoclaving the solution and packing  in our Class 100 Aseptic Facility.

Cell Culture Suited Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W110101)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

RNase and DNase Free Grade Buffer

PurMa Biologics manufactures this grade of buffer by using :PurMa™ Cell Culture Suited Water (Cat# P3W120103)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.2 µm filter and pack under N2

Cell Culture Suited / Endotoxin Depleted Grade Buffer

PurMa Biologics manufactures this grade of buffer by using: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)

In our Class 100 Aseptic Facility we Buffer will finally sterilize the buffer by 0.1 µm filter and pack under N2

The Endotoxin Removing Procedure from Buffer

PurMa biologics has the most sophisticated endotoxin removal. For that we pass the buffer  through the PurMa^TM Endotoxin Elimination Column (Cat# P6H1216251). Furthermore, in our proprietary column we covalently conjugate Sepharose 6B   to an endotoxin-specific absorbent. we finally  eliminate the residual endotoxin by passing the unbound fraction three times through 0.1µm filters.

Features

• it has an important role in molecular biology as well as Cell Culture Suited.
• PurMa Biologics manufactures Tris Buffer from  Mg²⁺ and Ca ²⁺-free water.
• Free of RNAse /DNase
• Free of Proteases. It is rigorously tested for contaminating nonspecific endonuclease, exonuclease, and RNase activity.
• It is rigorously tested for contamination of endotoxin and mycoplasma.
• Is manufactured in several pH formats.
• Sterile filtered by 0.1µm.
• As it is packed under N_2, the pH won’t change over time before opening the bottle.

References

1. The structure of crystalline tris: a plastic crystal precursor, buffer, and acetylcholine attenuator. Rudman R, Eilerman D, La Placa SJ. Science. 1978 May 5;200(4341):531-3. doi:10.1126/ science. 644311. PMID: 644311.
2. Interactions of tris buffer with nucleotides: the crystal structure of tris(hydroxymethyl) methylammonium adenosine 5′-diphosphate dihydrate. Shakked Z, Viswamitra MA, Kennard O. Biochemistry. 1980 Jun 10;19(12):2567-71. doi: 10.1021/bi00553a005. PMID: 7397089.