Whole Tissue Cryoprotective Media (WTCM-D) with DMSO
Cryopreservation of tissues especially when a big portion is intended to be used is challenging. Additionally, the biggest obstacle is forming ice crystals inside the cells. This inevitably, cause volume expansion with the destruction of cell membranes and holes in loose connective tissue. Whole Tissue Cryoprotective Media (WTCM-D) with DMSO, preserve tissues during rapid freezing. This remarkably promotes formation of amorphous ice ( rather than crystal ice).
Description
Background
Cryopreservation of tissues especially when a big portion is intended to be used is challenging. Additionally, the biggest obstacle is forming ice crystals inside the cells. This inevitably, cause volume expansion with the destruction of cell membranes and holes in loose connective tissue. Whole Tissue Cryoprotective Media (WTCM-D) with DMSO, preserve tissues during rapid freezing. This remarkably promotes formation of amorphous ice ( rather than crystal ice).
PurMa™ WTCM-D has particularly overcome several challenges
- Liquid nitrogen has a low specific heat constant. When it contacts warm tissues or OCT it boils and forms a vapor barrier and acts as an insulator. As a result, the inner core of the tissue sample freezes slowly and unevenly. This is especially more challenging when the tissue sample is large. The cryoprotective agents in PurMa™ WTCM-D precents this to happen by penetrating rapidly inside the tissue.
- PurMa™ WTCM-D acts regardless of the size of the tissue. PurMa™ WTCM has high thermal conductivity. This, therefore, can allow liquid nitrogen for rapid and efficient cryoprotection.
- PurMaTM WTCM contains ingredients which makes it suitable for the preservation of rigid tissues.
Special Features
- Ready-to-Use.
- PurMaTM WTCM provides much higher cell viability after short/long-term cryoprotection,
- PurMaTM WTCM Prevents the formation of crystals in extracellular space. This, consequently, prevents cellular dehydration and resulting in less exposure to toxins from the non-recovering cells.
- Preserves morphological appearance as a standard factor of cell recovery.
- Tested for pH, osmolality, sterility, and endotoxin.
- Each Lot of PurMaTM WTCM is tested for pathogens and sterility. We evaluate each Lot as well for several criteria. Including survival (by Trypan Blue method), viability & performance (by MTT Test), and the recovery rate by Alamar Blue test.
- PurMaTM WTCM has been tested successfully for several commonly used tissue including rodent heart, brain, and kidney.
Guideline
for PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines
References
- Diffusion and distribution of dimethyl sulphoxide in the isolated guinea-pig taenia coli. Elford BC. J Physiol. 1970 Jul;209(1):187-208. doi: 10.1113/jphysiol.1970.sp009162.
- Cryopreservation of whole ovine ovaries with pedicles as a model for human: parameters of perfusion with simultaneous saturations by cryoprotectants. Isachenko et al. Clin Lab. 2015;61(3-4):415-20. doi: 10.7754/clin.lab.2014.140919. PMID:
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.2 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | Sodium pyruvate |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Size | 1 x 10 ml, 1 x 100 ml |
|---|
Quality Control
[vc-quality-control-tab]11 reviews for Whole Tissue Cryoprotective Media (WTCM-D) with DMSO
Only logged in customers who have purchased this product may leave a review.
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azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 21-Feb-2025 15:30:00
Ease of placing the order: ‘not given’
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
Review:
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Time of submission: 24-Feb-2025 13:50:14
Ease of placing the order: ‘not given’
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
Review:
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Time of submission: 27-Feb-2025 17:42:44
Ease of placing the order: ‘not given’
Anonymous –
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Time of submission: 05-Mar-2025 16:31:44
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michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 05-Mar-2025 17:29:13
Ease of placing the order: ‘not given’
Anonymous –
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Time of submission: 10-Mar-2025 14:32:38
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jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5