PurMaFectin™ Transfection Reagent
PurMaFectin™ Transfection Reagent contains highly advanced lipid nanoparticle technology. Also, it provides superior transfection performance with improved application outcomes and reproducible results. Furthermore, it delivers superior transfection efficiency and improved cell viability. Do not add antibiotics to the medium during transfection as this causes cell death and interferes with transfection efficiency.
Description
Background
PurMaFectin™ Transfection Reagent contains highly advanced lipid nanoparticle technology. Also, it provides superior transfection performance with improved application outcomes and reproducible results. Furthermore, PurMaFectin™ Transfection Reagent delivers superior transfection efficiency and improved cell viability. Do not add antibiotics to the medium during transfection as this causes cell death and interferes with transfection efficiency.
Special Features
- For the highest efficacy and the lowest dead cells, transfect cells at around 70~80% confluency. We strongly recommend starting the seeding by counting trypsinized cells so there will be high consistency among your experiments.
- Different cell types react differently to the number of passages when they are used for transfection. To minimize the impact of passage on efficiency of transfection, either use the cells with the same passage number. Alternatively, use at least two different concentrations of transfection reagent as control in new transfect experiments before the condition is optimized.
- For HeLa cells, high level transfection requires the presence of serum in the medium during transfection. The amount of required serum depends on sequence and the length of plasmid. The exact level of serum should be determined empirically.
- Endotoxin-contaminated DNA results in inefficient transfection and can cause high cellular toxicity. We use PurMaTM Endotoxin Elimination Column (Cat# P6H1216251). In this proprietary kit, Sepharose 6B covalently conjugates to an endotoxin-specific absorbent.
- Some serum-free medium might interfere with the transfection of cation ions and plasmid. We strongly recommend washing the cells with a conventional media such as DMEM prior to transfection. This will result in, eliminating serum can. Afterwards, you can add serum to the media 2 hours post transfection.
User Manual
For complete protocol, click here:PurMaFectin_User Manual
References
- Novel cationic liposomes for DNA-transfection with high efficiency and low toxicity. Paukku et al. Chem Phys Lipids. 1997 May 30;87(1):23-9. doi: 10.1016/s0009-3084(97)00020-0. PMID: 9219346.
- A new efficient method for transfection of neonatal cardiomyocytes using histone H1 in combination with DOSPER liposomal transfection reagent. Kott et al. Cell Mol Genet. 1998 Jul;24(4):257-61. doi: 10.1023/b:scam.0000007128.56413.31. PMID: 10410680.
Additional information
| Size | 1 x 1 ml, 1 x 10 ml |
|---|
11 reviews for PurMaFectin™ Transfection Reagent
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azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
Review:
Have you purchased:
Time of submission: 21-Feb-2025 15:30:00
Ease of placing the order: ‘not given’
Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
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Time of submission: 24-Feb-2025 13:50:14
Ease of placing the order: ‘not given’
lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
Review:
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Time of submission: 27-Feb-2025 17:42:44
Ease of placing the order: ‘not given’
Anonymous –
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Time of submission: 05-Mar-2025 16:31:44
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michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
Review:
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Time of submission: 05-Mar-2025 17:29:13
Ease of placing the order: ‘not given’
Anonymous –
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Time of submission: 10-Mar-2025 14:32:38
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jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5