R Cells Cryoprotective Media (RCCM) With DMSO
$40.00 – $43.00
PurMa™ R -Cell Cryoprotective Media (RCCM-D) performs highly for cryopreservation of reproductive cells such as Oocytes & Spermatozoa. It contains proprietary formulation including DMSO.
Description
PurMa™ R -Cell Cryoprotective Media (RCCM-D)
Background
PurMa™ R -Cell Cryoprotective Media (RCCM-D) performs highly for cryopreservation of reproductive cells such as Oocytes & Spermatozoa. It contains proprietary formulation including DMSO. Exposing cells to temperatures below 0°C without the aid of cryoprotectants has two key consequences:
- Osmotic gradient forms across the cell membrane, which causes “cell dehydration”, and
- Ice formation and abnormal concentration of solutes in the intracellular matrix, osmatic shock, and toxicity.
PurMa Biologics, with decades of experience in cutting-edge science, has manufactured PurMa™ R Cells Cryoprotective Media (RCCM) With DMSO which gives a high survival and recovery rate for reproductive cells.
There are several criteria that make DMSO a powerful cryoprotective agent
- PurMa™ R -Cell Cryoprotective Media (RCCM-D) provides strong molecular stability.
- Besides, facilitates a high non-conductor constant.
- It contains the basic properties, salts solubility particularly for anions. Additionally contains a propensity to act as the acceptor atom in hydrogen bonding.
- Especially, DMSO makes a hydrogen bond with water which is 30% stronger than between two water molecules.
- The proprietary formulation of PurMa™ RCCM-D allows the cryoprotective agent getting inside the cells instantly and lower cell death because of penetration/freezing.
PurMa™ R -Cell Cryoprotective Media (RCCM-D) penetrates inside the cells instantly and lower cell death.
Special Features for R Cells Cryoprotective Media (RCCM) With DMSO
- Ready-to-Use medium.
- Each Lot of our PurMaTM RCCM-D with DMSO goes through pathogens and sterility evaluations.
- We evaluate each Lot for survival test (by Trypan Blue method), viability & performance evaluation (by MTT Test), as well as for the rate of recovery by Alamar Blue test.
- R Cells Cryoprotective Media (RCCM) With DMSO results in the lowest cell loss during freezing and thawing as well as the highest cell recovery. Moreover, it provides over 66% survival and recovery rate after long term cryopreservation.
- Sterile (Passed through 0.2µm filters).
- Moreover, PurMaTM RCCM-D provides an exceptional cryopreservation for primary and commercial cell lines.
- Suitable for clinical applications.
Guideline R Cells Cryoprotective Media (RCCM) With DMSO
For PurMa Biologics guideline for cryoprotection, click here as well as in corresponding tab: PurMa™ Guideline for Cryoprotecting various cell lines
References
- Fertilization of human oocytes following cryopreservation; normal karyotypes and absence of stray chromosomes. Gook et al. Hum Reprod. 1994 Apr;9(4):684-91. doi:10.1093/ oxfordjournals .humrep .a138572.
- Cryopreservation of single human spermatozoa. Cohen et al. Hum Reprod. 1997 May;12(5):994-1001. doi: 10.1093/humrep/12.5.994.
Parameter | Specification |
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Appearance | Red, clear liquid |
pH | 7.2 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | Sodium pyruvate |
Indicator | Phenol red |
Mycoplasma Detection | Negative |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Room temperature |
Additional information
Size | 1 x 10 ml, 1 x 100 ml |
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