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Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX)

Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX)

(11 customer reviews)

Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life.  RPMI-1640-MAX significantly improves cell viability and growth. We manufacture several types of RPMI-1640-MAX based on your requirements.

SKU: N/A Categories: Roswell-Park Memorial Institute 1640, Cell Culture Media Tags: Cell Culture, MAX, RPMI, RPMI 1640, L-Alanyl-L-Glutamine, glucose, Phenol Red, Without Phenol Red, Mammalian Cells

Description

Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX) is a commonly used media for Mammalian cell culture.

Advantages

The advantages of RPMI-1640-MAX over RPMI-1640 are:

  • Higher PH Capacity
  • Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
  • Possess longer shelf life.
  • More importantly, minimizes toxic ammonia build-up.
  • Significantly improves cell viability and growth.
  • Remains stable across a wide range of temperatures.
  • Improves the culture of primary cells.

Mechanism

Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.

Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.

In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.

Contents

Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX) contains:

  • 5 mM sodium pyruvate
  • High glucose (4.5g/L)
  • Non-Essential Amino Acids
  • 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of RPMI-1640-MAX  based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
  • Phenol Red

RPMI-1640-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).

Formulation

For complete formulation click here: RPMI-1640 MAX Formulation

References

  1. Culture of normal human leukocytes. Moore GE, et al. 1967 Feb 20;199(8):519-24. PMID: 4960081
  2. Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. Dunne HW, et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.
  3. Long-term culture of human leukaemic leukocytes. Pössnerová V, et al. 1970;17(5):513-23. PMID: 5274436
Parameter Specification
Appearance clear liquid, Red liquid
pH 7.4 ± 0.1
Osmolality 275-360 mOsm/L
Endotoxin NMT< 2EU/mL
Mycoplasma Negative
Suitability Suitable for mammalian cell culture
Additive NA
Indicator NA
Sterility Tested Sterile filtered using 0.22 µm filter
Form Liquid
Shipping Condition Dry Ice

 

Additional information

Condition

RPMI 1640 MAX, Standard Formulation, RPMI 1640 MAX, Standard Formulation (ATCC formulation), RPMI 1640 MAX, w/o Phenol Red, RPMI 1640 MAX, w/o Sodium Bicarbonate, RPMI 1640 MAX, w/o Glucose, RPMI 1640 MAX, w/o Phenol Red, w/o Sodium Bicarbonate, RPMI 1640 MAX, With Low Glucose (1 g/L), RPMI 1640 MAX, With High Sodium Bicarbonate (3.7 g/L), RPMI 1640 MAX, With 15 mM HEPES (3.6 g/L), RPMI 1640 MAX, With 25 mM HEPES (5.9 g/L), RPMI 1640 MAX, With 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), RPMI 1640 MAX, With 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L, RPMI 1640 MAX, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), RPMI 1640 MAX, w/o Phenol Red, with 25 mM HEPES (5.9 g/L), RPMI 1640 MAX, w/o Phenol Red, High Sodium Bicarbonate (3.7 g/L)
Format

Liquid, Powder
Size

10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L

11 reviews for Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX)

  1. azadi@live.ca –

    Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
    Have you purchased: Yes
    Time of submission: 29-Jan-2025 23:10:30
    Ease of placing the order: 5/5

  2. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 21-Feb-2025 15:30:00
    Ease of placing the order: ‘not given’

  3. Gregory-Mullen@ouhsc.edu –

    Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
    Have you purchased: Yes
    Time of submission: 21-Feb-2025 15:46:22
    Ease of placing the order: 5/5

  4. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 24-Feb-2025 13:50:14
    Ease of placing the order: ‘not given’

  5. lrothblu@ouhsc.edu –

    Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
    Have you purchased: It is my first Purchase
    Time of submission: 24-Feb-2025 13:53:22
    Ease of placing the order: 5/5

  6. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 27-Feb-2025 17:42:44
    Ease of placing the order: ‘not given’

  7. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 16:31:44
    Ease of placing the order: ‘not given’

  8. michael-rudolph@ouhsc.edu –

    Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
    Have you purchased: Yes
    Time of submission: 05-Mar-2025 16:35:21
    Ease of placing the order: 5/5

  9. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 05-Mar-2025 17:29:13
    Ease of placing the order: ‘not given’

  10. Anonymous –

    Review:
    Have you purchased:
    Time of submission: 10-Mar-2025 14:32:38
    Ease of placing the order: ‘not given’

  11. jkurien@hausbio.com –

    Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
    Have you purchased: Yes
    Time of submission: 12-Mar-2025 12:34:09
    Ease of placing the order: 5/5

Only logged in customers who have purchased this product may leave a review.

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