Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX)
$31.61 – $586.18
Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX) contains L-Alanyl-L-Glutamine, therefore supplying a longer shelf life. RPMI-1640-MAX significantly improves cell viability and growth. We manufacture several types of RPMI-1640-MAX based on your requirements.
Description
Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX)
Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX) is a commonly used media for Mammalian cell culture.
Advantages
The advantages of RPMI-1640-MAX over RPMI-1640 are:
- Higher PH Capacity
- Moreover, it contains 4 mM L-Alanyl-L-Glutamine (0.869 g/L) instead of L-Glutamine and therefore:
- Possess longer shelf life.
- More importantly, minimizes toxic ammonia build-up.
- Significantly improves cell viability and growth.
- Remains stable across a wide range of temperatures.
- Improves the culture of primary cells.
Mechanism
Although L-glutamine is a vital amino acid, it degrades, resulting in the generation of toxic ammonia and pyrrolidine carboxylic acid. One way to minimize L-glutamine degradation in media is to gradually add these amino acids to your cell culture. However, keeping track of time to keep the amount of L-glutamine at the same level is tedious, if not impossible.
Alternatively, L-alanyl-L-glutamine can be used because it is much more stable in aqueous solutions. More importantly, it does not easily degrade and gradually releases aminopeptidases. As a result, L-glutamine and L-alanine are then used by the cells for protein production in the TCA cycle.
In addition to our Max formulation products, we manufacture PurMaTM GluaSup (Cat# P3S210 559) is a combination of L-alanyl-L-glutamine which prevents degradation of L-glutamine.
Contents
Roswell Park Memorial Institute 1640-MAX (RPMI-1640-MAX) contains:
- 5 mM sodium pyruvate
- High glucose (4.5g/L)
- Non-Essential Amino Acids
- 3.7 g/L Sodium Bicarbonate; PurMa Biologics Manufactures several types of RPMI-1640-MAX based on the amount of Sodium Bicarbonate and HEPES (PH capacity)
- Phenol Red
RPMI-1640-MAX contains no proteins, lipids, or growth factors. Therefore, it requires supplementation, commonly with 10% Fetal bovine Serum (FBS).
Formulation
For complete formulation click here: RPMI-1640 MAX Formulation
References
- Culture of normal human leukocytes. Moore GE, et al. 1967 Feb 20;199(8):519-24. PMID: 4960081
- Leukocytes as carriers in the transmission of bovine leukemia: certain morphologic and functional characteristics of cultured leukocytes from normal and leukemic cattle. Dunne HW, et al. Am J Vet Res. 1970 Apr;31(4):597-617. PMID: 5437104.
- Long-term culture of human leukaemic leukocytes. Pössnerová V, et al. 1970;17(5):513-23. PMID: 5274436
Parameter | Specification |
Appearance | clear liquid, Red liquid |
pH | 7.4 ± 0.1 |
Osmolality | 275-360 mOsm/L |
Endotoxin | NMT< 2EU/mL |
Mycoplasma | Negative |
Suitability | Suitable for mammalian cell culture |
Additive | NA |
Indicator | NA |
Sterility Tested | Sterile filtered using 0.22 µm filter |
Form | Liquid |
Shipping Condition | Dry Ice |
Additional information
Condition | RPMI 1640 MAX, Standard Formulation, RPMI 1640 MAX, Standard Formulation (ATCC formulation), RPMI 1640 MAX, w/o Phenol Red, RPMI 1640 MAX, w/o Sodium Bicarbonate, RPMI 1640 MAX, w/o Glucose, RPMI 1640 MAX, w/o Phenol Red, w/o Sodium Bicarbonate, RPMI 1640 MAX, With Low Glucose (1 g/L), RPMI 1640 MAX, With High Sodium Bicarbonate (3.7 g/L), RPMI 1640 MAX, With 15 mM HEPES (3.6 g/L), RPMI 1640 MAX, With 25 mM HEPES (5.9 g/L), RPMI 1640 MAX, With 15 mM HEPES (3.6 g/L), High Sodium Bicarbonate (3.7 g/L), RPMI 1640 MAX, With 25 mM HEPES (5.9 g/L), High Sodium Bicarbonate (3.7 g/L, RPMI 1640 MAX, w/o Phenol Red, with 15 mM HEPES (3.6 g/L), RPMI 1640 MAX, w/o Phenol Red, with 25 mM HEPES (5.9 g/L), RPMI 1640 MAX, w/o Phenol Red, High Sodium Bicarbonate (3.7 g/L) |
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Format | Liquid, Powder |
Size | 10 X 1000ml, 10 X 500ml, 1 x 500 ml, 6 x 500 ml, 1 x 1000 ml, 6 x 1000 ml, 1 x 10 L, 1 x 50 L |
Quality Control
[vc-quality-control-tab]Formulation
SDS
SDS
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