Trypsin EDTA Free 0.5%
Ultra-pure EDTA-free Trypsin at 0.5% for routine dissociation of adherent cells.
- Ultra-pure trypsin, a serine protease, for detaching adherent cells
- Supplied in HBSS, available with or without phenol red
- ✓Proudly made in the USA, manufactured in house
- ✓Certificate of Analysis available for every lot
- ✓Backed by decades of cell culture and protein biochemistry expertise
- ✓Bulk pricing, purchase orders, and custom formulations welcome
Description
Background
PurMa™ Trypsin EDTA Free 0.5% is a trypsin without EDTA — an ultra-pure trypsin solution used to gently dissociate adherent cells in mammalian cell culture. Trypsin is a serine protease that cleaves peptide bonds at lysine and arginine residues (except when followed by proline), releasing cells from the culture surface and from one another for passaging, counting, and downstream work.
Why Trypsin Without EDTA?
Many trypsin solutions include EDTA, a chelating agent that binds calcium and magnesium ions to speed up detachment. PurMa™ Trypsin EDTA Free 0.5% contains no EDTA, giving you a gentler, more controlled dissociation. It’s preferred when working with cells sensitive to EDTA or to calcium/magnesium depletion, or when you want to preserve divalent-cation-dependent surface proteins and functions.
Features and Benefits
- 10X ready-to-use stock — no additional ingredients needed. Dilute with PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W010101) to your working concentration.
- Contains no EDTA, calcium, magnesium, or heavy metals.
- Contains no serine protease inhibitors (DFP, TLCK, APMSF, AEBSF).
- Contains no phytates or phytic acid salts (e.g., myo-inositol) that can inhibit trypsin activity.
- Ultra-pure trypsin (HPLC-purified, 99% purity), sourced from bovine pancreas.
- Rigorously evaluated for yeast, mold, mycoplasma, and endotoxin.
Available Concentrations
PurMa Biologics produces trypsin in 0.05%, 0.25%, 0.5%, 2.5%, and 5% — both with EDTA and EDTA-free — in phenol red or phenol-red-free formats, so you can match the strength and indicator to your protocol.
Specifications
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.8 ± 0.1 |
| Osmolality | 275–360 mOsm/L |
| Endotoxin | NMT < 2 EU/mL |
| Mycoplasma | Negative |
| Sterility | Sterile filtered (0.22 µm) |
| Indicator | Phenol red |
| Form | Liquid |
| Suitability | Mammalian cell culture |
| Shipping | Room temperature |
References
- Razin S, et al. Adhesion of mycoplasmas to eukaryotic cells. Ciba Found Symp. 1981;80:98-118.
- Goldberg DM, Roy AD. Fate of trypsin and chymotrypsin in the human small intestine. Gut. 1969;10(6):477-83.
- Shapira E, Arnon R, Russell A. Specific immunoassay for quantitative determination of human trypsin. J Lab Clin Med. 1971;77(5):877-84.
Learn more: What Is Trypsin?
Additional information
| Condition | Trypsin 0.5%, Standard Formulation, Trypsin 0.5%, Without Sodium Bicarbonate, Trypsin 0.5%, Without Calcium Chloride, Trypsin 0.5%, Without Glucose |
|---|---|
| Size | 1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml |
| Phenol Red | With Phenol Red, Without Phenol Red |
Documents
Certificate of Analysis (COA): Available upon request for your specific order — email info@purmabiologics.com and we will send the current lot documentation.
Safety Data Sheet (SDS): Available upon request — email info@purmabiologics.com.
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