Trypsin EDTA Free 0.5%
PurMa™ Trypsin EDTA Free 0.05% contains ultra-pure trypsin. This enzyme is a member of the serine protease family. Initially produced in the pancreas, Trypsin is then subsequently secreted and activated in the intestines. Cell culture laboratories use trypsin to dissociate adherent cells for routine cell culture.
PurMa™ Trypsin in HBSS is available with, as well as without EDTA in phenol red or without phenol red.
Description
Background
PurMa™ Trypsin EDTA Free 0.05% contains ultra-pure trypsin. This enzyme is a member of the serine protease family. Trypsin, initially produced in the pancreas, is subsequently secreted and activated in the intestines. Cell culture laboratories use trypsin to dissociate adherent cells for routine cell culture.
Trypsin Activity
Trypsin cleaves its substrates: IGDKLIPA unless these amino acids are followed by proline. PurMa™ trypsin is HPLC purified at 99% purity for bovine pancreas. Moreover, optical rotatory dispersion of trypsin in the presence of calcium shows a major conformational transition between 40 and 45° which results in deactivation of trypsin. Therefore, all the buffers needed to be Ca+2 & Mg+2 free for the proper function of this enzyme.
Preparation
The activity of this enzyme is further modulated by the sulfation of Tyr154. To overcome the mentioned issue, we use our most sophisticated water quality to ensure the absence of Mg+2 and Ca+2 as well as other heavy metals, please see: PurMa™ Ultra-Pure Cell Culture Suited Water (Cat# P3W110102)
Additionally, we include 2mM EDTA as it Chelates metals such as calcium, magnesium, lead, mercury, copper, iron…
PurMa™ Trypsin Deactivates Mycoplasma in your Cell Culture
Trypsin treatment abolishes the ability of mycoplasmas to attach to the cells. Incidentally, mycoplasma is a common, slowly deteriorating contamination in cell culture, especially due to the invisibility of the contamination. PurMa Biologics manufactures all the reagents you need to fight mycoplasma in your cell culture. The presence of a trace of SDS and other detergents leads to a decrease in the trypsin activity.
Features and Benefits
- PurMa TM Trypsin-EDTA Free 0.5% is a complete 10X Ready-To-Use stock solution and DOES NOT need any additional ingredients. Dilute with PurMaTM water; Cat number: P3W010101 to desired concentration and use.
- PurMa TM Trypsin-EDTA Free 0.5% does not contain calcium or magnesium ions or any other heavy metals.
- PurMa TM Trypsin-EDTA Free 0.5% does not contain serine protease inhibitors, including DFP, TLCK, APMSF, and AEBSEF.
- PurMa™ Trypsin Free does not contain Phytates or salts of phytic acid, such as myo-inositol which is commonly present in cell culture media and inhibits trypsin activity.
- PurMa™ Trypsin free has been rigorously evaluated for yeast, mold, mycoplasma, and endotoxin contamination.
Concentrations
PurMa Biologics produces 0.05%, 0.25%,0.5%, 2.5%, and 5% Trypsin with EDTA as well as without EDTA in phenol red or without phenol red. Moreover, we manufacture PurMa™ Trypsin in Hanks’ Buffer with 2mM EDTA (pH 7.8).
Formulation
Here is the complete formulation: 0.5% Trypsin without EDTA
References
Adhesion of mycoplasmas to eukaryotic cells. Razin et al. Ciba Found Symp. 1981;80:98-118. doi: 10.1002/9780470720639.ch8. PMID: 6790254.
Fate of trypsin and chymotrypsin in the human small intestine. Goldberg et alRoy AD. 1969 Jun;10(6):477-83. doi: 10.1136/gut.10.6.477. PMID: 5786145.
Specific immunoassay for quantitative determination of human trypsin in intestinal content. Shapira E, Arnon R, Russell A. J Lab Clin Med. 1971 May;77(5):877-84. PMID: 4997546han HL. J Biomed Sci. 2010 May 11;17(1):36. doi: 10.1186/1423-0127-17-36. PMID: 20459778.
| Parameter | Specification |
|---|---|
| Appearance | Red, clear liquid |
| pH | 7.8 ± 0.1 |
| Osmolality | 275-360 mOsm/L |
| Endotoxin | NMT< 2EU/mL |
| Mycoplasma | Negative |
| Suitability | Suitable for mammalian cell culture |
| Additive | EDTA |
| Indicator | Phenol red |
| Mycoplasma Detection | Negative |
| Sterility Tested | Sterile filtered using 0.22 µm filter |
| Form | Liquid |
| Shipping Condition | Room temperature |
Additional information
| Condition | Trypsin 0.5%, Standard Formulation, Trypsin 0.5%, Without Sodium Bicarbonate, Trypsin 0.5%, Without Calcium Chloride, Trypsin 0.5%, Without Glucose |
|---|---|
| Size | 1 x 100 ml, 6 x 100 ml, 1 x 500 ml, 6 x 500 ml |
| Phenol Red | With Phenol Red, Without Phenol Red |
11 reviews for Trypsin EDTA Free 0.5%
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azadi@live.ca –
Review: we have purchased Lipid Depleted FBS , DMEM F12 …. they were good quality and decent price
Have you purchased: Yes
Time of submission: 29-Jan-2025 23:10:30
Ease of placing the order: 5/5
Anonymous –
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Time of submission: 21-Feb-2025 15:30:00
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Gregory-Mullen@ouhsc.edu –
Review: We buy *all* of our cell culture media, fetal bovine sera, and lipid-depleted sera from PurMa. The quality of their reagents is absolutely top-notch, giving us reliable, repeatable results in our experiments. We highly recommend PurMa!
Have you purchased: Yes
Time of submission: 21-Feb-2025 15:46:22
Ease of placing the order: 5/5
Anonymous –
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Time of submission: 24-Feb-2025 13:50:14
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lrothblu@ouhsc.edu –
Review: Serum worked beautifully. Cells grew well and had their usual cycle time. Tissue culture medium also worked very well.
Have you purchased: It is my first Purchase
Time of submission: 24-Feb-2025 13:53:22
Ease of placing the order: 5/5
Anonymous –
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Time of submission: 27-Feb-2025 17:42:44
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Anonymous –
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Time of submission: 05-Mar-2025 16:31:44
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michael-rudolph@ouhsc.edu –
Review: I have worked directly with PurMa biologics on a number of projects ongoing in my lab. I find the media and the specialty Lipid Depleted Serum to be high quality and very consistent among batches, which is critical to our adipogenesis projects using primary stem cells. Also, the service from PurMa is outstanding for my lab.
Have you purchased: Yes
Time of submission: 05-Mar-2025 16:35:21
Ease of placing the order: 5/5
Anonymous –
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Time of submission: 05-Mar-2025 17:29:13
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Anonymous –
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Time of submission: 10-Mar-2025 14:32:38
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jkurien@hausbio.com –
Review: We have been working with PurMa biologics for the past several years , we buy cell culture related media like DMEM F12 and EMEM and Fetal Bovine Serum . We appreciate the high quality and reasonable price, and great customer service.
Have you purchased: Yes
Time of submission: 12-Mar-2025 12:34:09
Ease of placing the order: 5/5